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使用圆二色性光谱和傅里叶变化红外光谱研究冬小麦麸皮抗冻蛋白的二级结构

发表时间:2008-06-15  浏览量:2002  下载量:600
全部作者: 张超,张晖,赵晓燕,马越,姚惠源
作者单位: 北京市农林科学院国家蔬菜工程技术研究中心;江南大学食品科学与工程国家重点实验室
摘 要: 本文使用圆二色性光谱和傅里叶变化红外光谱技术对冬小麦麸皮抗冻蛋白的二级结构进行研究。由于冬小麦麸皮抗冻蛋白在酰胺I带和Ⅲ带位置中,各个特征峰相互叠加,因此采用Gausse峰型对圆头峰进行拟合、解析、判断其α-helix,β-sheet,β-turn和random coil的相对含量,并讨论了两种检测方法结果差异的原因,为冬小麦麸皮抗冻蛋白抗冻机理的研究奠定基础。
关 键 词: 分析化学;冬小麦麸皮抗冻蛋白;二级结构;傅里叶变化红外光谱;圆二色性光谱;酰胺I带和Ⅲ带
Title: The secondary structure of winter-wheat-bran antifreeze protein determined by FT-IR and CD spectrum
Author: ZHANG Chao, ZHANG Hui, ZHAO Xiaoyan, MA Yue, YAO Huiyuan
Organization: National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences;Jiangnan University, Key Laboratory of Food Science and Technology
Abstract: The secondary structure of the winter-wheat-bran antifreeze protein was analyzed by the fourier transform infrared (FT-IR) and circular dichroism (CD) spectrum. The smooth peak of the amide I and amide III region from the FT-IR profile was overlapped by the characteristic peak. So the characteristic peak of the α-helix, β-sheet, β-turn and random coil of winter-wheat-bran antifreeze protein was resolved. The content of the α-helix, β-sheet, β-turn and random coil was the accumulation of the characteristic peak respectively. Moreover, the secondary structure was evaluated by the CD spectrum. Finally, the difference between the FT-IR and CD spectrum results was discussed.
Key words: analytical chemistry; winter-wheat-bran antifreeze protein; secondary structure; fourier transform infrared spectrum; circular dichroism spectrum; amide Ⅰ and Ⅲ
发表期数: 2008年10月第11期
引用格式: 张超,张晖,赵晓燕,等. 使用圆二色性光谱和傅里叶变化红外光谱研究冬小麦麸皮抗冻蛋白的二级结构[J]. 中国科技论文在线精品论文,2008,1(11):1266-1271.
 
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