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酸枣仁汤对老年阴血亏虚型失眠证候模型大鼠学习记忆及脑内自由基、NOS的影响

发表时间:2008-08-15  浏览量:1503  下载量:733
全部作者: 游秋云,吴丽丽,田代志,王平
作者单位: 湖北中医学院药学院;湖北中医学院药学院
摘 要: 目的:探讨酸枣仁汤对老年阴血亏虚失眠证候模型大鼠学习记忆、脑组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性的影响。方法:用D-半乳糖致衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺睡眠,制作老年阴血亏虚型失眠证候大鼠复合模型,观察大鼠学习记忆、大脑皮质、海马部位MDA含量及SOD、NOS活性的变化,并考察酸枣仁汤对这些指标的影响。结果:与环境对照组比较,证候模型组睡眠剥夺48 h后,大鼠学习记忆能力显著下降,脑组织MDA含量和SOD、NOS活性均升高;经酸枣仁汤干预后大鼠学习记忆能力得以明显改善,脑内各指标明显下降(P<0�01,P<0�05),并趋于正常范围。结论:酸枣仁汤对老年阴血亏虚失眠证候模型大鼠的学习记忆能力障碍有改善作用,且酸枣仁汤大剂量组作用略优于小剂量组,其机理之一可能与减轻睡眠剥夺中自由基和NO对神经细胞的损伤有关。
关 键 词: 中药学;睡眠剥夺;模型;酸枣仁汤;学习记忆;自由基
Title: Effects of Suanzaoren Tang on learning memory and levels of free radical and NOS in old model rats brain with Yin and blood-deficiency and sleep deprivation
Author: YOU Qiuyun, WU Lili, TIAN Daizhi, WANG Ping
Organization: College of Pharmacy, Hubei University of Chinese Medicine;Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription
Abstract: Objective: To observe the effects of Suanzaoren Tang(SZRT) on learning memory and the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and nitric oxide synthase (NOS) in old model rats brain with Yin and blood-deficiency and sleep deprivation (SD). Methods: Levels of learning memory and MDA, and activities of SOD and NOS in mice brain were assayed after SD induced by self-made multiple platform method (MMPM), senescence induced by D-galactose, and Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum. Results: After 48 h SD, ability of learning and memory of rats decreased significantly, levels of MDA, and activities of SOD and NOS in mice brain markedly increased in comparison to the control group. However, the observation of the groups treated with SZRT significantly decreased (P<0.01, P<0.05), and showed a tendency in returning to normal leve1. Conclusion: SZRT can improve the disability of learning and memory of the pathogeny rats model, and one of its mechanisms maybe involve the reduction of nerve cell damage with free radical and NO. What’s more, the effect of high dose of SZRT is better than that of the low one.
Key words: science of Chinese materia medica; sleep deprivation (SD); model; Suanzaoren Tang(SZRT); learning memory; free radical
发表期数: 2008年12月第15期
引用格式: 游秋云,吴丽丽,田代志,等. 酸枣仁汤对老年阴血亏虚型失眠证候模型大鼠学习记忆及脑内自由基、NOS的影响[J]. 中国科技论文在线精品论文,2008,1(15):1728-1732.
 
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