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狼疮灵颗粒的提取工艺研究
发表时间：2009-12-15 浏览量：1519 下载量：507
| 全部作者： | 朱晗，冯俭，李煌，张梦云，张煜华，孙川 |
| 作者单位： | 成都中医药大学药学院 |
| 摘 要： | 目的：保证狼疮灵颗粒制剂工艺的可行性、合理性与稳定性，探讨其最佳提取工艺。方法：采用高效液相色谱（high performance liquid chromatography，HPLC）法测定芍药苷含量［测定条件：流动相为乙腈-0.4%的磷酸水溶液（体积比为13∶87）；检测波长230 nm；流速1.0 mL/min；柱温25℃］；采用硫酸-蒽酮法测定总多糖含量，并以干浸膏得率、总多糖含量和芍药苷含量为指标，采用L9（34）正交实验，用综合评分法处理数据优选水提取工艺。结果：经方差分析优选出的最佳制备工艺条件是A3B1C2，即加8倍量水，煎煮3次，每次1 h.结论：该提取工艺稳定，简便可行，重复性较好。 |
| 关 键 词： | 中药学；提取工艺；正交实验；狼疮灵颗粒 |
| Title： | Study on extraction process of Langchuangling granules |
| Author： | ZHU Han, FENG Jian, LI Huang, ZHANG Mengyun, ZHANG Yuhua, SUN Chuan |
| Organization： | School of Chinese Pharmacy, Chengdu University of Traditional Chinese Medicine |
| Abstract： | Objective: To ensure feasibility, rationality and stability of the preparation process, and investigate the extraction process of Langchuangling granules. Methods: Determining paeoniflorin content by high performance liquid chromatography (HPLC) (measurement conditions: mobile phase of acetonitrile -0.4% phosphoric acid solution (volume ratio 13∶87); detection wavelength 230 nm; flow rate 1.0 mL/min; and column temperature 25℃); and determining polysaccharide content by anthrone-sulfuric acid method. The extraction procedure of Langchuangling granule was measured, and the evaluated indexes were the rate of dry extra, contents of the paeoniflorin and the total polysaccharide with L9 (34) orthogonal test and preferential treatment of data. Results: The optimal process was performed by 1-hour extraction for three times, with 8-fold volume of water. Conclusion: The process is convenient, stable and reasonable. |
| Key words： | traditional Chinese medicine; extraction process; orthogonal test; Langchuangling granule |
| 发表期数： | 2009年12月第23期 |
| 引用格式： | 朱晗，冯俭，李煌，等. 狼疮灵颗粒的提取工艺研究[J]. 中国科技论文在线精品论文，2009，2（23）：2538-2541. |
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