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N/2/3DS肝素抑制小鼠黑色素瘤细胞B16F10转移研究

发表时间:2010-04-15  浏览量:1636  下载量:471
全部作者: 沈剑,郑胜,刘妍,王瑞飞,曾宪录
作者单位: 东北师范大学遗传与细胞研究所
摘 要: 分析了经化学修饰的肝素抗凝活性与抗肿瘤转移活性及其发挥抗肿瘤作用的机制。结果表明:经过化学修饰后得到的N/2/3DS肝素的抗凝血活性显著降低。进一步分析发现:N/2/3DS 肝素能够显著降低黑色素瘤细胞B16F10的肺转移。层流实验结果表明:N/2/3DS 肝素能够显著降低B16F10与血小板之间的黏附作用。N/2/3DS 肝素抑制肿瘤转移活性的发挥是通过抑制纤维蛋白原介导的肿瘤细胞与血小板之间的粘附作用实现的。研究结果提示:N/2/3DS肝素有可能作为肿瘤转移抑制药物用于临床研究。
关 键 词: 细胞生物学;肿瘤转移;层流粘附检测;N/2/3DS肝素;B16F10;抗凝血;纤维蛋白原
Title: Research on inhibition of mice melanoma cell B16F10 metastasis by N/2/3DS heparin
Author: SHEN Jian, ZHENG Sheng, LIU Yan, WANG Ruifei, ZENG Xianlu
Organization: Institute of Genetics and Cytology, Northeast Normal Univeristy
Abstract: This paper analyzes the anti-coagulation activity, anti-metastasis effect and mechanism of chemically modified heparins. It is found that modified N/2/3DS heparin has reduced anti-coagulation activity and it can retain the strong inhibitory effect on tumor metastasis. Under shear stress condition, heparin and N/2/3DS heparin reduce the number of melanoma cell B16F10 adhering to platelets. The experiments show that fibrinogen promotes the adhesion of B16F10 to platelets, and N/2/3DS heparin inhibits this effect. The results suggest that it is possible that N/2/3DS heparin can be used as anti-metastasis drug in clinic.
Key words: cytobiology; tumor metastasis; laminar flow adhesion assays; N/2/3DS heparin; B16F10; anti-coagulation; fibrinogen
发表期数: 2010年4月第7期
引用格式: 沈剑,郑胜,刘妍,等. N/2/3DS肝素抑制小鼠黑色素瘤细胞B16F10转移研究[J]. 中国科技论文在线精品论文,2010,3(7):655-659.
 
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