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猪miR-29c的克隆、组织表达谱及与部分免疫性状的关联分析
发表时间：2011-06-15 浏览量：1551 下载量：739
| 全部作者： | 黄菁，谢胜松，苏立杰，赵书红 |
| 作者单位： | 华中农业大学农业动物遗传育种与繁殖教育部重点实验室&农业部猪遗传育种重点开放实验室 |
| 摘 要： | 研究利用stem-loop RT-PCR法克隆猪miR-29c成熟序列，通过实时定量PCR技术检测出猪miR-29c在心、肝、脾、肺、肾、肌肉、胃、小肠等8种组织中广泛表达，其中在心脏、脾脏、肾脏中高表达。测序发现在miR-29c前体序列上游32 nt处有一个T/C突变，利用PCR-限制性片段长度多态性（restricted fragment length polymorphisms, RFLP）技术进行多态性检测及部分血液参数和免疫性状关联分析。结果显示：NcoⅠ酶切基因型与长白猪群体的出生重、0日龄（出生）的平均红细胞体积（mean red cell volume，MCV）、17日龄的血小板计数分布宽度（platelet distribution width，PLDW）和32日龄猪瘟抗体阻断率存在显著相关（P<0.05）；与0日龄的平均血红蛋白量（mean corpuscular hemoglobin，MCH）、0日龄和32日龄的平均血红蛋白浓度（mean corpuscular hemoglobin concentration， MCHC）存在极显著相关（P<0.01）。位于前体的单核苷酸多态性（single nucleotide polymorphism，SNP）可能调节Drosha的加工过程，从而影响miRNA的表达水平。 |
| 关 键 词： | 动物遗传育种与繁殖；猪；miR-29c；组织表达；性状关联分析 |
| Title： | Association analysis on cloning, organization expression pattern and part immune traits of porcine miR-29c |
| Author： | HUANG Jing, XIE Shengsong, SU Lijie, ZHAO Shuhong |
| Organization： | Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University |
| Abstract： | In this study, the mature sequence of porcine miR-29c was cloned by stem-loop RT-PCR. It is widely expressed in 8 pig tissues, including heart, liver, spleen, lung, kidney, muscle, stomach, and small intestine by real-time PCR assays. One T/C polymorphism at the position 32 nt of miR-29c precursors was tested in a Landrace population by PCR-RFLP and analyzed association with immune traits. The results show that NcoⅠ enzyme genotype is significantly associated (P<0.05) with birth weight, the mean red cell volume (MCV) of 0-day-old pigs; the platelet distribution width (PLDW) of 17-day-old pigs and block of classical swine fever antibodies of 32-day-old pigs, and is more significantly associated (P<0.01) with the mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) of 0-day-old pigs, the MCHC of 32-day-old pigs. The single nucleotide polymorphism in precursor can modulate Drosha processing efficiency and thereby affect the level of miRNA expression. |
| Key words： | animal genetics, breeding and reproduction; porcine; miR-29c; tissues expression; traits association analysis |
| 发表期数： | 2011年6月第11期 |
| 引用格式： | 黄菁，谢胜松，苏立杰，等. 猪miR-29c的克隆、组织表达谱及与部分免疫性状的关联分析[J]. 中国科技论文在线精品论文，2011，4（11）：1033-1038. |
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