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板栗疫病菌核糖体蛋白相关基因RPS18,RPL30,RPL32-B的克隆及其功能研究

发表时间:2012-10-15  浏览量:1257  下载量:506
全部作者: 李峰,林海燕,朱超,施李鸣,商巾杰,陈保善
作者单位: 广西大学生命科学与技术学院;广西大学亚热带农业生物资源保护与利用国家重点实验室;广西大学微生物及植物遗传工程教育部重点实验室
摘 要: 尝试对受低毒病毒调控的核糖体蛋白(ribosomal protein, RP)RPS18,RPL30和RPL32-B 3个基因分别进行基因敲除,但仅RPL32-B得到为数不多的转化子,且只能以杂合子的形式存在,表明这些核糖体基因在板栗疫病菌(Cryphonectria parasitica)中可能是必需的。构建了多核糖体基因RNA干扰质粒pFCNH-intron-sll并导入板栗疫病菌。核糖体RNA干扰导致宿主真菌致病力明显降低,表明RPS18,RPL30和RPL32-B或其中之一参与板栗疫病菌致病力的调节。�
关 键 词: 植物病理学;板栗疫病菌;核糖体蛋白;基因敲除;多基因RNA干扰;致病力�
Title: Study on cloning and functional characterization of the ribosomal protein-related genes RPS18, RPL30 and RPL32-B in Cryphonectria parasitica
Author: LI Feng, LIN Haiyan, ZHU Chao, SHI Liming, SHANG Jinjie, CHEN Baoshan
Organization: College of Life Science and Technology, Guangxi University; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University; Key Laboratory of Ministry of Education of China for Microbial and Plant Genetic Engineering, Guangxi University
Abstract: In this paper, three ribosomal protein (RP)-related genes RPS18, RPL30 and RPL32-B, whose expression are regulated by hypovirus infection, were knocked out respectively. However, only knockout mutants for RPL32-B were obtained. These mutants were heterozygous that could not be further purified to homozygous, demonstrating that these three RP genes were indispensable in Cryphonectria parasitica. An RNAi silencing recombinant plasmid pFCNH-intron-sll targeting RPS18, RPL30 and RPL32-B was developed and introduced into C. parasitica. Transformants showed significant reduction in virulence to chestnut stems, demonstrating that one or all of RPS18, RPL30 and RPL32-B was involved in virulence regulation in C. parasitica.�
Key words: phytopathology; Cryphonectria parasitica; ribosomal protein; gene knockout; multi-gene RNA silencing; virulence
发表期数: 2012年10月第19期
引用格式: 李峰,林海燕,朱超,等. 板栗疫病菌核糖体蛋白相关基因RPS18,RPL30,RPL32-B的克隆及其功能研究[J]. 中国科技论文在线精品论文,2012,5(19):1841-1849.
 
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