您的位置：首页 > 论文页面
鲍鱼腹足酸性多糖的分离纯化、组成分析及其体外抗肿瘤活性研究
发表时间：2012-11-15 浏览量：1865 下载量：667
| 全部作者： | 李国云，杜磊，常耀光，薛勇，薛长湖，王玉明 |
| 作者单位： | 中国海洋大学食品科学与工程学院 |
| 摘 要： | 对鲍鱼腹足肌酸性粘多糖（abalone acid polysaccharide，AAP）进行提取、分离纯化及基本组成分析，并研究其体外抗肿瘤活性。鲍鱼腹足肌经木瓜蛋白酶酶解提取、氯化十六烷基吡啶（CPC）沉淀、盐溶、乙醇沉淀、透析、冻干得到鲍鱼腹足肌粗多糖，经DEAE离子交换层析和S-300凝胶过滤层析后得到一种纯度较高的新型AAP. 经鉴定其平均分子量（molecular weight，MW）为56.2 ku；柱前衍生高效液相色谱法分析单糖组成，绝大部分为氨基半乳糖（GalN）、葡萄糖醛酸（GlcUA）、岩藻糖（Fuc）和半乳糖（Gal），其物质的量之比为2.14∶2.37∶2.97∶1，此外还均含少量鼠李糖（Rha）和氨基葡萄糖（GlcN）；离子色谱法测定硫酸基含量为15.5%. 体外抗肿瘤试验表明，AAP能够显著抑制小鼠S180肉瘤细胞和人肝癌细胞HEP-G2的增殖。 |
| 关 键 词： | 水产品加工与贮藏；鲍鱼；酸性多糖；分离纯化；化学组成；体外抗肿瘤 |
| Title： | Isolation, chemical composition and antitumor activity in vitro of the acid polysaccharide isolated from Haliotis discus hannai |
| Author： | LI Guoyun, DU Lei, CHANG Yaoguang, XUE Yong, XUE Changhu, WANG Yuming |
| Organization： | College of Food Science and Technology, Ocean University of China |
| Abstract： | A novel glycosaminoglycan-like abalone acid polysaccharide (AAP) from the pleopods of Haliotis discus hannai was purified by DEAE ion exchange chromatography followed with S-300 HR gel filtration chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14∶2.37∶2.97∶1, the content of sulfate was 15.5%. The average molecular weight (MW) was 56.2 ku in a TSK G4000 column. The antitumor activity of the sulfated polysaccharide in vitro was detected by MTT assay. It was found that AAP had an obvious antitumor activity in a dose-dependent manner in vitro and the maximum inhibitory ratio of S180 and HEP-G2 were 52.5% (800 mg/L) and 48.9% (800 mg/L), respectively. |
| Key words： | aquatic products processing and storage; abalone; acid polysaccharide; isolation and purification; chemical composition; antitumor |
| 发表期数： | 2012年11月第21期 |
| 引用格式： | 李国云，杜磊，常耀光，等. 鲍鱼腹足酸性多糖的分离纯化、组成分析及其体外抗肿瘤活性研究[J]. 中国科技论文在线精品论文，2012，5（21）：2104-2109. |
0
评论数 0请您登录
暂无评论
下载全文
多维论文