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TAK-778通过雌激素受体依赖通道诱导去卵巢大鼠的成骨能力
发表时间：2013-06-15 浏览量：1491 下载量：367
| 全部作者： | 刘鹏程，蔡明 |
| 作者单位： | 同济大学附属第十人民医院骨科 |
| 摘 要： | 目的：使用去卵巢(ovariectomized, OVX)大鼠模型检测TAK-778是否依赖雌激素受体（estrogen receptor，ER）通路发挥诱导成骨作用。方法：OVX大鼠造模2周后口服TAK-778和/或他莫昔芬（tamoxifen）3月余，溶液组（vehicle）和假手术组（sham）作为对照组。测量腰椎骨矿物密度（bone mineral density，BMD）、L3椎体矢状二维图、骨形成率（bone formation rates，BFR）、血清钙及骨钙素水平。结果：与vehicle相比，TAK-778显著提高了BMD、血清钙和骨钙素及BFR水平。研究还发现，使用雌激素受体拮抗剂tamoxifen可以明显抑制TAK-778诱导的上述指标的增加；Micro-CT扫描显示经TAK-778处理的OVX大鼠的骨结构模型指数(structure model index, SMI)、骨体积/组织体积(bone volume/tissue volume, BV/TV)和骨小梁厚度(trabecular thickness, Tb.Th)增加，骨小梁间距(trabecular separation/spacing, Tb.Sp)缩小；tamoxifen与TAK-778结合抑制了上述观察指标。结论：TAK-778通过雌激素依赖受体介导的信号通路增强OVX大鼠的成骨能力。 |
| 关 键 词： | 外科学；成骨；TAK-778；他莫昔芬；雌激素受体；去卵巢大鼠 |
| Title： | TAK-778 induces osteogenesis in ovariectomized rats through an estrogen receptor-dependent pathway |
| Author： | LIU Pengcheng, CAI Ming |
| Organization： | Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University |
| Abstract： | Objective: To test if TAK-778 induces osteogenesis through an estrogen receptor (ER) dependent pathway using an ovariectomized (OVX) rat model. Methods: Two weeks after test animals underwent ovariectomy, TAK-778 and/or tamoxifen was administered orally over 3 months. Vehicle-treated and sham-operated rats were served as controls. The bone mineral density (BMD) of the lumbar vertebrae and sagittal two-dimensional images of the L3 vertebral body were measured. In addition, bone formation rates (BFR) and serum calcium and osteocalcin levels were investigated. Results: TAK-778 significantly increased BMD, serum calcium and osteocalcin levels, and BFR when comparing to those of the vehicle-treated group. However, tamoxifen clearly inhibited the increase in these parameters induced by TAK-778. In addition, micro-CT tomography scanning showed that treatment with TAK-778 increased the structure model index (SMI), bone volume/tissue volume (BV/TV), and trabecular thickness (Tb.Th) parameters, while decreased the trabecular spacing (Tb. Sp) in OVX rats. Tamoxifen suppressed these effects when was combined with TAK-778. Conclusion: Taken together, the present study showed that TAK-778 enhanced osteogenesis in ovariectomized rats through an estrogen receptor-dependent pathway. |
| Key words： | surgery; osteogenesis; TAK-778; tamoxifen; estrogen receptor; ovariectomized rats |
| 发表期数： | 2013年6月第11期 |
| 引用格式： | 刘鹏程，蔡明. TAK-778通过雌激素受体依赖通道诱导去卵巢大鼠的成骨能力[J]. 中国科技论文在线精品论文，2013，6（11）：1027-1032. |
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