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农用抗生素KA08活性组分分离及类型分析

发表时间:2013-10-15  浏览量:1219  下载量:492
全部作者: 高芬,李静虹,王梦亮
作者单位: 山西大学应用化学研究所
摘 要: 采用薄层层析色谱法(thin layer chromatography,TLC),以硅胶GF254为固定相,以乙醇:水=6:4和乙醇:氨水:水=8:1:1(体积比)为展开剂,结合生物追踪测定,分离纯化农用抗生素KA08的活性组分。获得的活性组分IA和IC,经高效液相色谱(high performance liquid chromatography,HPLC)检测,纯度分别为97.53%和98.79%. 通过官能团反应、紫外和红外吸收光谱分析,推测这2种组分均属于氨基糖苷类抗生素,其中,组分IC是一类相对分子量为269.0的小分子化合物。
关 键 词: 植物保护学;抗生素KA08;薄层层析;分离纯化;类型分析
Title: Isolation of active components and analysis of structural types in agro-antibiotic KA08
Author: GAO Fen, LI Jinghong, WANG Mengliang
Organization: Institute of Applied Chemistry, Shanxi University
Abstract: Thin layer chromatography (TLC) combined with bioassay were applied to the isolation of the active components in agro-antibiotic KA08. The silica gel plate (GF254) was used as the stationary phase, and two mixed solvents, one containing ethanol and water in the volume ratio of 6:4, and the other ethanol, aqueous ammonia and water in the volume ratio of 8:1:1, were used as the developing reagents. The purity of active component IA and IC was 97.53% and 98.79% by high performance liquid chromatography (HPLC) respectively. The functional groups’ reaction, UV and IR spectrum showed that components IA and IC most likely belonged to aminoglycoside antibiotic. Component IC was a small molecule compound with molecular weight of 269.0.
Key words: plant protection; agro-antibiotic KA08; thin layer chromatography; isolation and purification; analysis of structural type
发表期数: 2013年10月第19期
引用格式: 高芬,李静虹,王梦亮. 农用抗生素KA08活性组分分离及类型分析[J]. 中国科技论文在线精品论文,2013,6(19):1807-1813.
 
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