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款冬花提取物抗氧化活性及对小肠细胞α-葡萄糖苷酶抑制作用研究

发表时间:2013-10-15  浏览量:1264  下载量:297
全部作者: 张岩,王洁,张俊,黄毅娜,高鸿
作者单位: 四川大学轻纺与食品学院;四川大学华西公共卫生学院
摘 要: 研究款冬花提取物(crude extract of Tussilago farfara,CET)中多酚类物质的抗氧化活性,并用人类结肠癌(Caco-2)细胞模型分析CET对小肠α-葡萄糖苷酶活性的抑制作用。实验测得CET中总酚与总黄酮含量分别为(116±0.82)mg没食子酸当量/g提取物与(181±3.89)mg芦丁当量/g提取物。经高效液相色谱(high performance liquid chromatography,HPLC)分析,得到绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸及芦丁为CET中主要的活性物质。CET清除2,2’-联氨-双(3-乙基苯并噻唑啉-6磺酸)二胺盐(ABTS)自由基、丁苯酞对1,1-二苯代苦味肼(DPPH)自由基的半数有效浓度(EC50)分别为(1.254±0.091)g/L,(0.055±0.016)g/L. 将提取物作用于经H2O2处理过的Caco-2细胞,平均细胞活力提高38.58%. CET(2.5 g/L)作用于Caco-2细胞单层模型,对细胞绒毛面顶侧(apical,AP)和基底侧(basolateral,BL)麦芽糖酶活性的抑制率分别为(56.84±1.03)%和(51.68±1.39)%. 该结果表明:款冬花可作为一种潜在的资源应用于抗氧化和抗餐后高血糖保健食品的开发。
关 键 词: 食品科学技术基础学科;款冬花;抗氧化;糖尿病;α-葡萄糖苷酶;Caco-2细胞
Title: Antioxidant and α-glucosidase inhibitory activities of Tussilago farfara L. flower bud extracts
Author: ZHANG Yan, WANG Jie, ZHANG Jun, HUANG Yina, GAO Hong
Organization: College of Light Industry, Textile and Food Engineering, Sichuan University; College of Huaxi Public Health, Sichuan University
Abstract: This study was performed to investigate antioxidant of the phenols in crude extract of Tussilago farfara (CET) and α-glucocidase inhibitory activities using Caco-2 cell model. The total phenols and flavonoids contents were (116±0.82) mg gallic acid equivalent/g CET and (181±3.89) mg rutin equivalent/g CET, respectively. Chlorogenic acid, 3,4-dicafeoylquinic acid, 3,5-dicafeoylquinic acid, 4,5-dicafeoylquinic acid and rutin were detected as the main active compounds of CET by high performance liquid chromatography (HPLC) analysis. CET exhibited a potent scavenging effect on 2,2’-azino-bio (3-ethylbenz-thiazoline-6-sulphonic acid) free radical (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) with EC50 values of (1.254±0.091) g/L and (0.055±0.016) g/L, respectively. CET exhibited a protecting effect on Caco-2 cells when treating with H2O2. This extract showed an inhibitory effect against the maltase enzyme with (56.84±1.03)% inhibition on apical (AP) side and (51.68±1.39)% inhibition on basal (BL) side of Caco-2 monolayer at a concentration of 2.5 g/L. These results suggested that T.farfara could be used as a potential antidiabetic and antioxidation material in food industry.
Key words: basic subject of food science and technology; Tussilago farfara L.; antioxidant; diabetic; α-glucosidase; Caco-2 cell
发表期数: 2013年10月第19期
引用格式: 张岩,王洁,张俊,等. 款冬花提取物抗氧化活性及对小肠细胞α-葡萄糖苷酶抑制作用研究[J]. 中国科技论文在线精品论文,2013,6(19):1893-1900.
 
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