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TRB3与糖尿病肾病肾小管细胞凋亡的关系

发表时间:2014-06-15  浏览量:1706  下载量:718
全部作者: 王伟伟,吕莎莎,程静,刘向春,关广聚
作者单位: 山东大学医学院;山东大学第二医院肾内科
摘 要: 目的:探讨tribble 3(TRB3)在糖尿病肾病(diabetic nephropathy,DN)大鼠肾脏中的表达及与肾小管凋亡的关系。方法:40只雄性Wistar大鼠,随机分成对照组、糖尿病(diabetes mellitus,DM)组、DM+空白病毒组和DM+TRB3-siRNA病毒组。腹腔注射链脲菌素(streptozotocin,STZ)诱导DM模型,尾静脉注射慢病毒沉默TRB3的表达。定期监测血糖、体重及尿白蛋白排泄(urinary albumin excretion,UAE)。免疫组化、实时定量RT-PCR及Western blotting检测TRB3在肾脏的表达,原位末端标记(in situ terminal deoxynucleotidyl transferase-dUTP nick-end labeling,TUNEL)法及Caspase 3活性测定法检测肾脏细胞的凋亡。结果:与对照组相比,DM大鼠肾脏TRB3表达量明显增加,免疫组化检测到TRB3表达在肾小管细胞,而肾小球未见表达。TUNEL法在对照组大鼠的肾脏中极少检测到凋亡细胞,而DM大鼠的肾小管细胞出现明显凋亡。Caspase 3活性检测结果表明DM大鼠肾脏Caspase 3的活性较对照组明显增强。用TRB3-siRNA沉默TRB3的表达后,肾小管细胞的凋亡数明显减少且Caspase 3活性明显降低。结论:TRB3在DM大鼠的肾脏中表达明显升高,而且介导了肾小管细胞的凋亡。
关 键 词: 内科学;糖尿病肾病;凋亡;基因沉默;TRB3
Title: TRB3 mediates apoptosis of renal tubular cells in diabetic nephropathy
Author: WANG Weiwei, LÜ Shasha, CHENG Jing, LIU Xiangchun, GUAN Guangju
Organization: Medical School, Shandong University; Department of Nephrology, The Second Hospital of Shandong University
Abstract: Objective: To research the expression of tribble 3 (TRB3) in the kidney of diabetic nephropathy (DN) rats and the relationship between TRB3 expression and apoptosis of renal tubular cells. Methods: 40 male Wistar rats were randomly divided into the control group, diabetes mellitus (DM) group, DM+vehicle group and DM+TRB3-siRNA group. The DM rat model was induced by streptozotocin (STZ) injection in abdominal cavity, and intravenous injection of lentivirus silenced the TRB3 expression. The body weight, blood glucose and urinary albumin excretion (UAE) were regularly monitored.The immunohistochemical, RT-PCR and Western blotting were used to check TRB3 expression in kidney. In situ terminal deoxynucleotidyl transferase-dUTP nick-end labeling (TUNEL) and Caspase 3 activity measurement were used to check the apoptosis of tubular cells. Results: Compared with the control, TRB3 expression was obviously upregulated in kidneys of diabetic rats. Immunohistochemical staining localized TRB3 expression in renal tubules, but not in glomeruli. TUNEL staining showed that apoptotic cells were rarely observed in kidneys of control rats, but significantly increased in tubules of diabetic kidneys. In addition, Caspase 3 activity was markedly higher in kidneys of diabetic rats compared with the control. However, the number of apoptotic tubular cells and Caspase 3 activity were significantly decreased after TRB3 silencing. Conclusion: TRB3 is upregulated in kidneys of diabetic rats and mediates apoptosis of renal tubular cells.
Key words: internal medicine; diabetic nephropathy; apoptosis; gene silencing; TRB3
发表期数: 2014年6月第11期
引用格式: 王伟伟,吕莎莎,程静,等. TRB3与糖尿病肾病肾小管细胞凋亡的关系[J]. 中国科技论文在线精品论文,2014,7(11):1031-1037.
 
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