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没食子儿茶素没食子酸酯降低P糖蛋白表达逆转人耐药鼻咽癌细胞株多药耐药的研究

发表时间:2014-06-15  浏览量:1938  下载量:785
全部作者: 朱美婵,谢貌,石书婧,李晓雪,祁承林,李恒,易翔,唐安洲
作者单位: 广西医科大学第一附属医院耳鼻咽喉头颈外科
摘 要: 目的:探讨绿茶中的儿茶素单体没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)对人耐药鼻咽癌(nasopharyngeal carcinoma,NPC)细胞HNE1/DDP株多药耐药性(multi-drug resistance, MDR)的逆转作用及相关机制。方法:采用MTT法确定EGCG的非细胞毒性剂量,以及对HNE1/DDP细胞药物敏感性的影响。同时,采用荧光实时定量PCR(quantitative real-time PCR, QRT-PCR)及Western blotting方法分别检测其产物P糖蛋白(P-glycoprotein,P-gp)的表达。结果:EGCG在低于154.6 μmol/L时对HNE1/DDP细胞的生长抑制率小于10%. 60,80,120 μmol/L的EGCG均可增加HNE1/DDP细胞对顺铂的敏感性,使顺铂对HNE1/DDP细胞的50%生长抑制率浓度(50% inhibition concentration,IC50)由原来的4.22 mg/L降低至3.82,2.56,1.78 mg/L,其逆转倍数分别为1.10,1.65和2.37倍。Western blotting结果显示,不同浓度的EGCG均可抑制HNE1/DDP细胞P-gp的表达。结论:EGCG可部分逆转人耐药NPC细胞株HNE1/DDP对顺铂的耐药性,其逆转机制与增加细胞内化疗药物浓度、抑制P-gp的表达有关。
关 键 词: 肿瘤学;鼻咽癌;没食子儿茶素没食子酸酯;P糖蛋白
Title: Multidrug resistance-reversing effect of epigallocatechin gallate on human nasopharyngeal carcinoma cell lines by decreasing expression of P-glycoprotein
Author: ZHU Meichan, XIE Mao, SHI Shujing, LI Xiaoxue, QI Chenglin, LI Heng, YI Xiang, TANG Anzhou
Organization: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University
Abstract: Objective: To evaluate the value of epigallocatechin gallate (EGCG) on the multi-drug resistance (MDR) reversing effect human nasopharyngeal carcinoma (NPC) HNE1/DDP cell lines and its possible mechanisms. Methods: Cytotoxicity of EGCG and its sensibility to cis-platinum in HNE1/DDP cell line, were evaluated by MTT assay. Furthermore, the expression of P-glycoprotein (P-gp) was detected by quantitative real-time PCR (QRT-PCR) and Western blotting respectively. Results: The 10% inhibition concentration of EGCG to HNE1/DDP cell line was 154.6 μmol/L. Therefore, 60, 80, 120 μmol/L of EGCG were used to detect the reversal effect of MDR. 50% inhibition concentration (IC50) for DDP was 4.22 mg/L. EGCG could increase the drug susceptibility of HNE1/DDP cell to DDP, and the IC50 for DDP decreased to 3.82, 2.56, 1.78 mg/L, and the modulating factors were 1.10, 1.65 and 2.37 fold respectively. The expressions of P-gp in HNE1/DDP cells incubated with EGCG were deceased when detected by QRT-PCR and Western blotting. Conclusion: EGCG could partially reverse the multidrug resistance of the NPC HNE1/DDP cells in vitro. Moreover, the reversal effect may be due to the increase of intracellular accumulation of chemotherapeutic drugs and the decrease of the expression of P-gp.
Key words: oncology; nasopharyngeal carcinoma; epigallocatechin gallate; P-glycoprotein
发表期数: 2014年6月第11期
引用格式: 朱美婵,谢貌,石书婧,等. 没食子儿茶素没食子酸酯降低P糖蛋白表达逆转人耐药鼻咽癌细胞株多药耐药的研究[J]. 中国科技论文在线精品论文,2014,7(11):1044-1048.
 
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