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携带bFGF生长因子的NCP/PLLA复合材料在原位软骨修复中的应用

发表时间:2014-06-15  浏览量:1798  下载量:530
全部作者: 黄鑫,师钟丽,翁文剑,杨迪生
作者单位: 浙江大学医学院附属第二医院骨科;浙江大学材料科学与工程学系
摘 要: 目的:研究携带碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的新型纳米α/β相磷酸钙(nano-α/β calcium phosphate,NCP)/聚乳酸(poly-L-lactic acid,PLLA)复合材料在体内软骨缺损修复中的作用。方法:雄性家兔12只,在双侧股骨内侧髁上做一直径4 mm、深4 mm的全层软骨缺损,在一侧缺损内植入携带bFGF的NCP/PLLA材料,另一侧缺损设置为空白对照。术后4周和8周,通过大体和病理学评价软骨修复情况。结果:4周后,实验组软骨缺损被白色再生组织修复,镜下观察发现大量的未分化细胞和干细胞填充在材料孔内,同时伴有成簇样软骨基质形成。8周后,缺损被成熟的软骨组织修复,并伴有大量的软骨基质形成。而在对照组,不论是4周还是8周,缺损内只填充有纤维组织。结论:携带有bFGF的NCP/PLLA新型复合材料可有效修复软骨缺损。
关 键 词: 骨外科学;软骨修复;组织工程;成纤维细胞生长因子
Title: In situ cartilage repair using NCP/PLLA scaffold incorporated with bFGF
Author: HUANG Xin, SHI Zhongli, WENG Wenjian, YANG Disheng
Organization: Department of Orthopaedics, The Second Affiliated Hospital, School of Medicine, Zhejiang University; Department of Material Science and Engineering, Zhejiang University
Abstract: Objective: To evaluate the effort of the novel nano-α/β calcium phosphate (NCP) /poly-L-lactic acid (PLLA) incorporated with basic fibroblast growth factor (bFGF) as the carrier for articular cartilage regeneration in vivo. Methods: Full-thickness defects (diameter 4 mm×4 mm in depth) were created in the medial femoral condyles in 12 adult male rabbits. The defect in one side of condyle was filled with NCP/PLLA scaffold with bFGF mixture and another was left without treatment as a control. Macrography and histological analysis were evaluated after 4 and 8 week postoperation. Results: At 4 week postoperation, the defects in experimental group were filled with white regenerated tissue. Histological analysis showed that abundant of undifferentiated cells or immature cartilaginous cells were present in the micropores with clusters of cartilaginous extracellular matrix formation. At 8 week postoperation, well-formed and mature cartilage was resurface the defects. Whereas only fibrous tissue replacement was observed for the control either at 4 or 8 weeks. Conclusion: The novel NCP/PLLA incorporated with bFGF can effectively treat the cartilage defects.
Key words: bone surgery; cartilage regeneration; tissue engineering; basic fibroblast growth factor
发表期数: 2014年6月第11期
引用格式: 黄鑫,师钟丽,翁文剑,等. 携带bFGF生长因子的NCP/PLLA复合材料在原位软骨修复中的应用[J]. 中国科技论文在线精品论文,2014,7(11):1061-1065.
 
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