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维甲酸对 CD2AP基因mRNA水平表达的影响

发表时间:2014-06-15  浏览量:1671  下载量:722
全部作者: 邱灵芝,高珊,朱亮华,庄丽丽,周国平
作者单位: 南京医科大学第一附属医院儿科
摘 要: 目的:研究旨在探讨9顺式维甲酸(9-cis retinoic acid,9-cis RA)、13顺式维甲酸(13-cis RA)、他米巴罗汀(AM80)对CD2相关蛋白(CD2-associated protein,CD2AP)基因mRNA水平表达的影响。方法:9-cis RA,13-cis RA及AM80作用人近端肾小管上皮细胞(HK-2)、人胚肾293(HEK-293)细胞后,用实时荧光定量PCR(real-time PCR,RT-PCR)检测CD2AP基因mRNA表达水平。结果:9-cis RA上调HK-2,HEK-293细胞CD2AP基因mRNA水平;13-cis RA,AM80不能上调HK-2,HEK-293细胞CD2AP基因mRNA水平。结论:在HK-2,HEK-293细胞中,9-cis RA能在转录水平上调CD2AP基因表达,而13-cis RA,AM80不能。
关 键 词: 儿科学;维甲酸;CD2AP;mRNA
Title: Effect of retinoic acid on the expression of CD2AP gene mRNA level
Author: QIU Lingzhi, GAO Shan, ZHU Lianghua, ZHUANG Lili, ZHOU Guoping
Organization: Department of Pediatrics, The First Affiliated Hospital, Nanjing Medical University
Abstract: Objective: To investigate the effect of 9-cis retinoic acid (9-cis RA), 13-cis RA and tamibarotene (AM80) on the expression of CD2AP (CD2-associated protein) gene mRNA level. Methods: Total RNA prepared from HK-2 and HEK-293 cells, with or without 9-cis RA,13-cis RA, AM80, was subjected to real-time PCR (RT-PCR). Results: The level of CD2AP transcripts increased with 9-cis RA treatment in HK-2 and HEK-293 cells. But 13-cis RA and AM80 could not increase the expression of CD2AP gene mRNA level in both HK-2 and HEK-293 cells. Conclusion: 9-cis RA may have a therapeutic effect on the kidney disease with a decreased expression of CD2AP, while the 13-cis RA, AM80 have no similar effect.
Key words: pediatrics; retinoic acid; CD2AP; mRNA
发表期数: 2014年6月第11期
引用格式: 邱灵芝,高珊,朱亮华,等. 维甲酸对 CD2AP基因mRNA水平表达的影响[J]. 中国科技论文在线精品论文,2014,7(11):1066-1070.
 
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