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硅酸盐细菌代谢产物防治实验性石棉肺研究

发表时间:2014-06-15  浏览量:1763  下载量:758
全部作者: 王晔,杨晨璐,程薇波,刘慧,赵雨佳,刘慧冉
作者单位: 四川大学华西公共卫生学院
摘 要: 目的:研究硅酸盐细菌代谢产物对大鼠吸入石棉纤维后的保护作用。方法:健康雄性SD大鼠80只,随机分为正常对照组5只、干预对照组5只、模型组35只、干预组35只;模型组与干预组各分为1 d,3 d,1周,2周,4周,8周,10周组,共7个亚组,每亚组5只。对于模型组各亚组动物,采用超声雾化石棉粉尘的方法建立大鼠石棉肺模型。对于干预组动物,除建模外,同时采用超声雾化硅酸盐细菌代谢产物给药,每日0.5 h.对于正常对照组动物,仅雾化蒸馏水使之吸入;而对干预对照组动物,仅雾化硅酸盐细菌代谢产物,使之吸入;各组动物分别于相应时间段处死。取肺组织进行病理学检查,并取血液与肺组织的丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)测定,同时检测肺组织羟脯胺酸(hydroxyproline,HYP)的含量,对实验结果进行统计学分析。结果:正常对照组未见明显异常,干预对照组偶见有轻微的炎症反应,其余与正常对照组相似;模型组早期以急性炎症为主,随即转为慢性炎症,伴随纤维组织增生,随时间延长而加剧;干预组则炎症反应轻微,仅有少许纤维组织增生。与正常对照组相比,干预对照组肺及血清SOD,MDA,HYP均无统计学差异。模型组与干预组的SOD,MDA,HYP均高于正常对照组,但干预组MDA,HYP水平明显低于模型组,而SOD水平明显高于模型组。结论:硅酸盐细菌代谢产物能在一定程度上减缓石棉肺的发生发展。
关 键 词: 职业病学;石棉肺预防;硅酸盐细菌代谢产物;实验性研究
Title: Study on effect of silicate bacteria metabolites on the experimental asbestosis
Author: WANG Ye, YANG Chenlu, CHENG Weibo, LIU Hui, ZHAO Yujia, LIU Huiran
Organization: West China School of Public Health, Sichuan University
Abstract: Objective: To detect the protective effect of the silicate bacteria metabolites on the rats that have inhaled the asbestos. Methods: 80 healthy male rats were divided into normal control group (n=5), treatment control group (n=5), asbestos-inhaled group (n=35) and asbestos-inhaled-and-treated group (n=35). The asbestos-inhaled group and asbestos-inhaled-and-treated group were also divided into 7 subunit groups i.e 1 d, 3 d, 1-week, 2-week, 4-week, 8-week, 10-week group, 5 rats in each group. For each subunit group of asbestos-inhaled group, the asbestos lung model was established by using the method of ultrasonic atomization asbestos dust. For treatment group, besides for model establishment, at the same time, the rats were treated drug with ultrasonic atomization metabolites of silicate bacterium. For normal group, the rats were only with the inhalation of nebulized distilled water. For treatment and control group, the rats were only with the inhalation of the atomization of silicate bacteria metabolites. Each group rats were sacrificed at the corresponding day. The lung and blood samples were extracted for pathological examination. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and hydroxyproline (HYP) were detected. The results were analyzed by statistics software. Results: Normal control group looked normal. Treatment control group was similar to the normal control group, and slight inflammatory reaction could be seen occasionally. Mild acute inflammation could be seen in the asbestos-inhaled group at early stage, then it converted to chronic inflammation with fibrous tissue hyperplasia. As time went on, the fibrosis became more serious. In the asbestos-inhaled-and-treated group, inflammatory reaction was very slight and only a little fibrous tissue hyperplasia could be seen. There were no significant difference of the levels of the MDA, SOD, HYP between the normal control group and treatment control group, as well as the lung index. However, the SOD, MDA, HYP of asbestos-inhaled group and asbestos-inhaled-and-treateel group were higher than those of normal control group, while the MDA, HYP of asbestos-inhaled-and-treated group were lower than those of asbestos-inhaled group, but SOD was higher in the former. Conclusion: The silicate bacteria metabolites could slow down the development of asbestosis in a certain extent.
Key words: occupational medicine; treatment of asbestosis; silicate bacterial metabolites; experimental research
发表期数: 2014年6月第11期
引用格式: 王晔,杨晨璐,程薇波,等. 硅酸盐细菌代谢产物防治实验性石棉肺研究[J]. 中国科技论文在线精品论文,2014,7(11):1071-1079.
 
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