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珍稀濒危种——斧形沙芥种子发育过程中脱水耐性研究
发表时间：2014-10-15 浏览量：1835 下载量：476
| 全部作者： | 孔德娟，张凤兰，郝丽珍，杨忠仁，田沐荣 |
| 作者单位： | 内蒙古农业大学农学院，内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室 |
| 摘 要： | 探讨斧形沙芥（Pugionium dolabratum Maxin.）种子脱水耐性的获得及其与抗氧化系统之间的相关性。将发育过程中的斧形沙芥种子经脱水处理，测定其含水量、萌发率与抗氧化系统相关指标。结果表明：斧形沙芥种子在40~60开花后天数（days after flowering, DAF），含水量逐渐下降，干重增加；50 DAF种子具有萌发能力，萌发率为20%；脱水可促进斧形沙芥种子的萌发，且人工脱水至含水量为12%和5%时，萌发率分别为55%和42%；发育过程中斧形沙芥种子过氧化物酶（peroxidase, POD）活性逐渐降低，而超氧化物歧化酶（superoxide dismutase, SOD）、过氧化氢酶（catalase, CAT）、抗坏血酸过氧化物酶（ascorbate peroxidase, APX）及脂肪氧化酶（lipoxidase, LOX）活性均呈上升趋势；脱水后，随着DAF的增加，POD活性逐渐降低，CAT和LOX活性逐渐升高，而SOD活性呈先降低后升高的变化趋势。经脱水和未脱水的斧形沙芥种子，其丙二醛（malondialdehyde, MDA）含量和超氧阴离子（O-2）产生速率均随DAF的增加而增加，但50 DAF和60 DAF的种子在脱水过程中的增加幅度小于40 DAF. 斧形沙芥种子在50 DAF时已获得脱水耐性，且脱水耐性强于沙芥种子。 |
| 关 键 词： | 蔬菜学；斧形沙芥种子；发育过程；脱水耐性；抗氧化系统 |
| Title： | Pugionium dolabratum Maxin. seeds during the development |
| Author： | KONG Dejuan, ZHANG Fenglan, HAO Lizhen, YANG Zhongren, TIAN Murong |
| Organization： | Inner Mongolia Autonomous Region Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Agronomy, Inner Mongolia Agricultural University |
| Abstract： | The relationship between the desiccation tolerance of Pugionium dolabratum Maxin. seeds and the antioxidant system was analyzed. P.dolabratum Maxin. seeds during the development were dehydrated, and the changes of moisture contents, germination rate and relevant index to antioxidant system were measured. The results showed that: from 40 to 60 days after flowering (DAF), the moisture content of P.dolabratum Maxin. seeds decreased gradually, but the dry weight increased. At 50 DAF, the seeds could germinate and the germination rate was 20%. Dehydration enhanced the germination rate, when the moisture content of seeds decreased to 12% and 5% with silica gel dehydration, the germination rate was up to 55% and 42% respectively. The activity of peroxidase (POD) decreased gradually during the development of P.dolabratum Maxin. seeds, but the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and lipoxidase (LOX) increased. With the increase of the DAF in the dehydration process, the activity of POD decreased gradually, while the activity of CAT and LOX increased, but the activity of SOD decreased firstly and then increased. With the increase of the DAF, the content of malondialdehyde (MDA) and O-2 generation rate in P.dolabratum Maxin. seeds with dehydration and no dehydration increased, but the increased in 50 and 60 DAF seeds during the dehydration process were less than 40 DAF. The P.dolabratum Maxin. seeds had acquired desiccation tolerance at 50 DAF, and the desiccation tolerance was more compared with P.cornutum seeds. |
| Key words： | olericulture; Pugionium dolabratum Maxin.; development; desiccation tolerance; antioxidant system |
| 发表期数： | 2014年10月第19期 |
| 引用格式： | 孔德娟，张凤兰，郝丽珍，等. 珍稀濒危种——斧形沙芥种子发育过程中脱水耐性研究[J]. 中国科技论文在线精品论文，2014，7（19）：1949-1956. |
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