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‘酥梨’LTR反转录转座子整合酶序列预测及其聚类分析

发表时间:2015-11-15  浏览量:1811  下载量:448
全部作者: 蒋爽,蔡丹英,骆军,滕元文
作者单位: 浙江大学农业与生物技术学院,农业部园艺植物生长发育与品质调控重点开放实验室;上海市农业科学院林木果树研究所,上海市设施园艺技术重点实验室;浙江省农业科学院园艺研究所
摘 要: 为深入了解‘酥梨’长末端重复序列(long terminal repeated,LTR)反转录转座子内部结构域,基于生物信息学方法对‘酥梨’基因组中LTR反转录转座子内部不同类型的整合酶进行预测,共获得346条整合酶氨基酸序列。通过系统聚类,‘酥梨’中整合酶可分为7类。序列比对结果显示,整合酶序列的中部含有D*GGE保守区域,序列后部含有NG*AE保守区域,其他部分保守性低,说明‘酥梨’中整合酶具有较高的异质性。这预示着‘酥梨’基因组中LTR反转录转座子的复杂起源过程。
关 键 词: 果树学;整合酶;预测;酥梨;反转录转座子
Title: Prediction and evolution of integrase of LTR retrotransposons in ‘Suli’ pear (Pyrus pyrifolia white pear group)
Author: JIANG Shuang, CAI Danying, LUO Jun, TENG Yuanwen
Organization: State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development & Quality Improvement, College of Agriculture & Biotechnology, Zhejiang University; Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences
Abstract: In order to further understand the inner domain of the long terminal repeated (LTR) retransponsons in ‘Suli’ pear (Pyrus pyrifolia), different types of integrase in the whole genome of ‘Suli’ pear were predicted by bioinformatics methods. A total of 346 integrase amino acid sequences were obtained. The cluster analysis indicated that there were seven lineages of integrase in ‘Suli’ pear. Sequence alignment showed two conserved regions, one is D*GGE in the middle of the sequences, the other is NG*AE in the end. The main part of sequence are not conservative, suggesting a high heterogeneity in integrase sequences, which may indicate the origin of LTR retrotransposons in ‘Suli’ pear genome were complicated.
Key words: pomology; integrase; prediction; Pyrus pyrifolia; retrotransposons
发表期数: 2015年11月第21期
引用格式: 蒋爽,蔡丹英,骆军,等. ‘酥梨’LTR反转录转座子整合酶序列预测及其聚类分析[J]. 中国科技论文在线精品论文,2015,8(21):2237-2240.
 
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