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柑橘毛状根瞬时转化体系的建立

发表时间:2015-11-15  浏览量:2189  下载量:633
全部作者: 白福玺,潘志勇
作者单位: 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室
摘 要: 为克服柑橘遗传转化效率低、周期长等问题,利用发根农杆菌MSU440侵染枳胚根,诱导其形成毛状根,并通过转化载体表达的红色荧光蛋白快速筛选到转化根,建立了快速有效的柑橘毛状根瞬时转化体系。毛状根在荧光显微镜下发红色荧光,表明载体T-DNA区成功转入了柑橘根系中。本研究在2个月时获得了拥有转基因根系的柑橘嵌合体植株,且转化效率达到29.8%,为研究柑橘根系发育和菌根共生等相关基因的功能提供了快速有效的方法。
关 键 词: 果树学;柑橘;发根农杆菌;毛状根;瞬时转化
Title: Establishment of hairy root transient transformation system in citrus
Author: BAI Fuxi, PAN Zhiyong
Organization: Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University
Abstract: In order to overcome the low efficiency and long period of regeneration of citrus genetic transformation, Agrobacterium rhizogenes MSU440 was employed to infect the radicle of P. trifoliate, then hairy roots were induced and transformed roots were fast distinguished by the expression of red fluorescence protein. Hairy roots showed red fluorescence under fluorescence microscope, which illustrated that T-DNA region of the vector had been transformed to hairy root successfully. In this study, chimeric plants that contained transgenic roots were obtained in two months and the transformation efficiency was 29.8%, which provides a fast and efficient method for function analysis of genes related to citrus root development and symbiosis.
Key words: pomology; citrus; Agrobacterium rhizogenes; hairy root; transient transformation
发表期数: 2015年11月第21期
引用格式: 白福玺,潘志勇. 柑橘毛状根瞬时转化体系的建立[J]. 中国科技论文在线精品论文,2015,8(21):2247-2250.
 
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