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siRNA纳米微囊的制备及其初步研究

发表时间:2015-12-15  浏览量:1461  下载量:541
全部作者: 周俊辉,杨征,邓联东,董岸杰
作者单位: 天津大学化工学院
摘 要: 采用可逆加成-断裂链转移(reversible addition-fragmentation chain transfer,RAFT)自由基聚合方法合成了一系列2-(二异丙基氨基)甲基丙烯酸乙酯(DPA)含量不同的两亲性共聚物MPEG-b-P(TTMA -co-DPA).通过调节DPA的含量调节聚合物中pH值敏感单体TTMA的水解速率。采用双乳化溶剂蒸发法制备了负载小干扰RNA(small interfering RNA,siRNA)的纳米微囊。实验表明,双乳化的纳米微囊粒径均一且毒性较低,是一种前景光明的递送载体。
关 键 词: 有机高分子材料;纳米微囊;siRNA;TTMA;双乳化溶剂蒸发法
Title: Preparation and preliminary study of the siRNA-loaded polymer nanocapsules
Author: ZHOU Junhui, YANG Zheng, DENG Liandong, DONG Anjie
Organization: School of Chemical Engineering and Technology, Tianjin University
Abstract: In this paper, a series of amphiphilic copolymers MPEG-b-P bearing (2,4,6- trimethoxybenzy-lidene-1,1,1-tris(hydroxymethyl) ethane methacrylate (TTMA) and 2-di(isopropylamino) ethyl methacrylate (DPA) with different feed ratios were obtained by reversible addition-fragmentation chain transfer (RAFT) radical copolymerization. By adjusting the content of DPA, the hydrolysis rate of pH sensitive monomer TTMA in the polymer was regulated. The siRNA-loaded polymer nanocapsules were constructed by double emulsion solvent evaporation method. The experimental results show that the size of the nanocapsules is uniform and the toxicity is lower, which would make a promising delivery carrier.
Key words: organic macromolecule materials; nanocapsules; siRNA; TTMA; double emulsion solvent evaporation method
发表期数: 2015年12月第23期
引用格式: 周俊辉,杨征,邓联东,等. siRNA纳米微囊的制备及其初步研究[J]. 中国科技论文在线精品论文,2015,8(23):2454-2459.
 
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