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西达本胺对luminal A型乳腺癌细胞株MCF-7增殖抑制和诱导凋亡的机制研究

发表时间:2016-03-15  浏览量:1670  下载量:847
全部作者: 施秀青,马飞,李慧慧,王海娟,张雪燕,林晨,钱海利,徐兵河
作者单位: 北京协和医学院/中国医学科学院肿瘤医院肿瘤研究所肿瘤内科;中国医学科学院肿瘤医院肿瘤研究所肿瘤内科;中国医学科学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室
摘 要: 目的:研究组蛋白去乙酰化酶抑制剂(histone deacetylase inhibitor,HDACi)西达本胺对luminal A型乳腺癌细胞株MCF-7体外增殖及凋亡的作用。方法:MTS法观察不同浓度西达本胺(2,5,10,20,50,100 μmol/L)对MCF-7细胞的增殖抑制作用,处理细胞后采用倒置显微镜观察细胞形态,流式细胞仪检测细胞周期、凋亡及乳腺癌干细胞比例,Western blotting检测干预后细胞p53蛋白的表达水平。结果:西达本胺在体外能明显抑制MCF-7细胞的增殖,与剂量呈正相关(P<0.05),细胞形态发生显著改变,流式细胞仪检测显示细胞出现明显凋亡,且剂量越高、作用时间越长,凋亡率越高(P<0.05),细胞周期和干细胞比例无变化。西达本胺处理后细胞p53蛋白较对照组表达明显升高。结论:西达本胺能够明显抑制MCF-7细胞的生长并诱导其凋亡,其作用机制可能与调控p53蛋白的表达有关。
关 键 词: 肿瘤学;组蛋白去乙酰化酶抑制剂;西达本胺;乳腺癌;凋亡
Title: Effect of Chidamide on proliferation and apoptosis of luminal A breast cancer cell line MCF-7
Author: SHI Xiuqing, MA Fei, LI Huihui, WANG Haijuan, ZHANG Xueyan, LIN Chen, QIAN Haili, XU Binghe
Organization: Department of Medical Oncology, Cancer Hospital & Institute, Peking Union Medical College/ Chinese Academy of Medical Sciences; Department of Medical Oncology, Cancer Hospital & Institute, Chinese Academy of Medical Sciences; National Key Laboratory of Molecular Oncology, Cancer Hospital & Institute, Chinese Academy of Medical Sciences
Abstract: Objective: To investigate effect of histone deacetylase inhibitor (HDACi) Chidamide on proliferation and apoptosis of luminal A breast cancer MCF-7 cells in vitro. Methods: Human breast cancer MCF-7 cell line was treated with different concentrations of Chidamide (2, 5, 10, 20, 50, 100 μmol/L), and blank group was established as control. Cell growth was assessed by MTS. Morphological changes of MCF-7 cells were observed under inverse microscopy, and apoptosis, cell cycles as well as stem cell proportion were analyzed by flow cytometry assay. Western blotting was used to detect expression of p53 protein between experimental and control groups. Results: Chidamide was able to inhibit proliferation of MCF-7 in vitro in positive dosage dependent manner (P<0.05). After being treated with Chidamide, cell morphology appeared great changes. Flow cytometry assay showed obvious apoptosis compared with control group with the increase of drug concentration and treatment time (P<0.05), but no alternation of cell cycles and stem cell proportion were detected. The expression of p53 protein increased obviously after treatment compared with control group. Conclusion: Chidamide could significantly inhibit proliferation of MCF-7 cells, and induce its apoptosis via upregulation expression of p53 protein.
Key words: oncology; histone deacetylase inhibitor; Chidamide; breast cancer; apoptosis
发表期数: 2016年3月第5期
引用格式: 施秀青,马飞,李慧慧,等. 西达本胺对luminal A型乳腺癌细胞株MCF-7增殖抑制和诱导凋亡的机制研究[J]. 中国科技论文在线精品论文,2016,9(5):449-455.
 
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