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铀响应的DNA水凝胶设计合成及结合微流控芯片的距离可视化检测应用

发表时间:2016-07-15  浏览量:4424  下载量:2369
全部作者: 黄艺顺,方璐婷,朱志,杨朝勇
作者单位: 厦门大学化学化工学院
摘 要: 设计并制备了铀酰响应的智能水凝胶,并将其用于铀酰离子的快速、便携、灵敏检测。水凝胶由铀酰离子依赖的脱氧核酶序列及其底物链以碱基互补配对方式连接短链DNA接枝的聚丙烯酰胺链形成。脱氧核酶的酶链和底物链的复合物被用作水凝胶的交联剂。当样品中存在铀酰离子时,酶链被激活,切割底物链,降低交联剂的浓度使得水凝胶稳定性下降,释放出包埋的金纳米粒子。用这种比色检测的方法可实现对100 nmol/L铀酰离子的肉眼识别。更进一步,为克服可视化检测中肉眼观察存在误差难以定量的缺陷,将水凝胶中包埋的颗粒替换为铂金纳米粒子,可实现利用气动芯片(volumetric bar-chart chip,V-chip)对天然湖水中铀酰离子浓度进行定量检测的目的。该方法可用于野外无熟练操作人员及复杂仪器时对铀酰离子进行便携、定量检测。
关 键 词: 分析化学;便携检测;铀酰离子;脱氧核酶;DNA水凝胶;气动芯片
Title: Design and synthesis of uranium-responsive DNA hydrogel for distance-based visual detection with microfluidics
Author: HUANG Yishun, FANG Luting, ZHU Zhi, YANG Chaoyong
Organization: College of Chemistry and Chemical Engineering, Xiamen University
Abstract: In this paper, a uranyl ion (UO22+) responsive smart hydrogel was constructed for rapid, portable, sensitive detection of uranyl ion. The uranyl ion dependent DNAzyme composing of enzyme strand and substrate strand complex was utilized to crosslink the DNA grafting polyacrylamide chains to form DNA hydrogel. Colorimetric analysis was done by encapsulating gold nanoparticles (AuNPs) in the hydrogel to indicate the concentrations of uranyl ion. Without uranyl ion, the enzyme strand was not active. The presence of uranyl ion would trigger the cleaving reaction of enzyme strand to substrate strand which would decrease the density of crosslinkers to destabilize the hydrogel and thus release the encapsulated AuNPs. As low as 100 nmol/L uranyl ion was visually detected by naked eye. Furthermore, to avoid the visual errors which may be caused by naked eye observation, a previously developed volumetric bar-chart chip (V-chip) was used to quantitatively detect and verify the results of uranyl ion concentrations in natural water by encapsulating Au-Pt nanoparticles in the hydrogel. The method can be used for portable and quantitative uranyl ion quantitation in field applications without skilled operators and sophisticated instruments.
Key words: analytical chemistry; portable detection; uranyl ion; DNAzyme; DNA hydrogel; volumetric bar-chart chip
发表期数: 2016年7月第13期
引用格式: 黄艺顺,方璐婷,朱志,等. 铀响应的DNA水凝胶设计合成及结合微流控芯片的距离可视化检测应用[J]. 中国科技论文在线精品论文,2016,9(13):1327-1337.
 
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