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外周血单个核细胞Sirt1表达水平与类风湿关节炎病理进程相关性研究

发表时间:2016-09-15  浏览量:1395  下载量:377
全部作者: 甘 露,何乙锦,王衍堂,石桂秀
作者单位: 四川大学华西医院麻醉与危重急救研究室;河西学院附属张掖人民医院手术室;成都医学院基础医学院免疫教研室;厦门大学第一附属医院风湿免疫科
摘 要: 目的:探讨Sirtuins家族成员沉默交配型信息调节因子2同源蛋白(silent mating type information regulation 2 homdog 1,SIRT1)在类风湿关节炎(rheumatoid arthritis,RA)病理进程中的可能作用机制。方法:随机纳入RA初治患者(79人)及招募正常对照人群(58人),收集外周血并分离外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)。提取细胞总RNA,实时定量PCR(real-time quantitative PCR,RT-qPCR)检测PBMCs中Sirt1 mRNA表达水平,并使用SPSS与RA患者各项临床检测指标进行Spearman相关性分析。结果:相较于正常人群,RA患者PBMCs中Sirt1 mRNA表达水平显著降低,且与RA患者疾病活动性评分(DAS28)、类风湿因子(rheumatoid factor,RF)、C反应蛋白(C-reactive protein,CRP)和红细胞沉降速率(erythrocyte sedimentation rate,ESR)呈显著负性相关。结论:淋巴细胞中Sirt1表达的降低与RA病理进程密切相关,该研究为RA临床诊断和治疗提供了新的思路和方法。
关 键 词: 风湿病学与自体免疫病学;自身免疫性疾病;类风湿关节炎;Sirt1;外周血单个核细胞
Title: Correlation study between Sirt1 expression in the peripheral blood mononuclear cells with the pathological process of rheumatoid arthritis
Author: GAN Lu, HE Yijin, WANG Yantang, SHI Guixiu
Organization: Laboratory of Anesthesia and Critical Care Medicine, West China Hospital; Operating Room, Zhangye People’s Hospital Affiliated to Hexi University; Department of Immunology, School of Basic Medical Sciences, Chengdu Medical College; Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital, Xiamen University
Abstract: Objective: To investigate the potential effect of a Sirtuins family member, silent mating type information regulation 2 homolog 1 (SIRT1), in the pathological process of rheumatoid arthritis (RA). Methods: In the study, 79 RA patients and 58 age-/sex-matched healthy volunteers were randomly selected, and the peripheral blood mononuclear cells (PBMCs) were collected. The total RNA was extracted from PBMCs and the Sirt1 transcripts were analyzed by real-time quantitative PCR (RT-qPCR). The correlations between Sirt1 mRNA and clinical test indexes were confirmed by Spearman correlation analysis using SPSS softwere. Results: Compared to healthy volunteers, the Sirt1 transcripts were obviously reduced in PBMCs from RA patients. The results of Spearman correlation analysis showed that the Sirt1 mRNA expression of PBMCs from RA patients were negatively correlated with the DAS28, the serum rheumatoid factor (RF), C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) in RA patients. Conclusion: The reduced expression of Sirt1 is closely correlated with the physiological process of RA, which provides a novel insight for the diagnosis and therapy strategy of RA.
Key words: rheumatology and autoimmune diseases; autoimmune disease; rheumatoid arthritis; Sirt1; peripheral blood mononuclear cells
发表期数: 2016年9月第17期
引用格式: 甘 露,何乙锦,王衍堂,等. 外周血单个核细胞Sirt1表达水平与类风湿关节炎病理进程相关性研究[J]. 中国科技论文在线精品论文,2016,9(17):1789-1794.
 
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