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Pestalotiopsis coffeae真菌次级代谢产物的研究

发表时间:2016-09-15  浏览量:1638  下载量:506
全部作者: 王 忠,高玉静,陈 波,郑新鸽,罗都强
作者单位: 河北大学生命科学学院;河北大学药物化学与分子诊断教育部重点实验室
摘 要: 以内生真菌Pestalotiopsis coffeae为研究对象,对其大比例固体发酵产物采取硅胶柱色谱、Sephadex LH-20凝胶柱色谱、预制硅胶薄层层析、高效液相色谱法(high performance liquid chromatography,HPLC)以及重结晶等分离方法,再通过波谱学技术进行结构鉴定,实验共分离并鉴定了7个化合物,分别为:6,8-dihydroxy-3-methoxy-3,7-dimethylisochroman-1,4-dione、邻苯二甲酸二(4-甲代戊基)酯、邻苯二甲酸二异丁酯、(R)-5,7-dihydroxy-3-((S)-1-hydroxyethyl)isobenzofuran-1(3H)-one、(S)-5,7-dihydroxy-3-((S)-1-hydroxyethyl) isobenzofuran-1(3H)-one、(R)-5,7-dihydroxy-3-((S)-1-hydroxyethyl)-6-methylisobenzofuran-1(3H)-one、(S)-5,7-dihydroxy- 3-((S)-1-hydroxyethyl)-6-methylisoben-zofuran-1(3H)-one.
关 键 词: 生物药物学;内生真菌;次级代谢产物;生物活性
Title: Study on secondary metabolites of the fungus Pestalotiopsis cof-feae
Author: WANG Zhong, GAO Yujing, CHEN Bo, ZHENG Xinge, LUO Duqiang
Organization: College of Life Science, Hebei University; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University
Abstract: In this paper, we selected one endophytic fungi Pestalotiopsis coffeae to study on the secondary metabolites. We carried out large-scale rice solid fermentation for the strain of fungus, then we extracted and purified the products of fermentation by means of silica gel column chromatography, Sephadex LH-20 gel filtration chromatography, preparative thin layer chromatography, high performance liquid chromatography (HPLC), recrystallization and so on. Structures of the extracted compounds were identified by various modern spectroscopic techniques. Seven compounds were separated and identified from this experiment. The structures of the known compounds were 6,8-dihydroxy-3-methoxy-3,7-dimethylisochroman-1,4-dione, bis(4-methylpen-tyl)phthalate,diisobutyl phthalate, (R)-5,7-dihydroxy-3-((S)-1-hydroxyethyl)isobenzofuran- 1(3H)-one, (S)-5,7-dihydroxy-3-((S)-1-hydroxyethyl)isobenzofuran-1(3H)-one, (R)-5,7-dihydroxy-3-((S)-1- hydroxyethyl)-6-methylisobenzofuran-1(3H)-one, (S)-5,7-dihydroxy-3-((S)-1-hydroxyethyl)-6-methylisoben- zofuran-1(3H)-one.
Key words: biopharmacy; endophytic fungi; secondary metabolites; bioactivity
发表期数: 2016年9月第17期
引用格式: 王 忠,高玉静,陈 波,等. Pestalotiopsis coffeae真菌次级代谢产物的研究[J]. 中国科技论文在线精品论文,2016,9(17):1815-1819.
 
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