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NO/H2S双供体型丁苯酞衍生物8d对大鼠脑缺血再灌注损伤的保护作用及其机制

发表时间:2016-09-15  浏览量:1355  下载量:536
全部作者: 兰 丽,吉 敬,季 晖
作者单位: 中国药科大学药理学教研室
摘 要: 目的:研究经口服给予NO/H2S双供体型丁苯酞衍生物8d对大鼠脑缺血再灌注损伤的保护效应。方法:除伪手术组外的其他各组大鼠均采用线栓法闭塞大脑中动脉(middle cerebral artery occlusion,MCAO)制备大鼠局灶性脑缺血再灌注模型。通过神经功能学评分、脑梗死面积测定、脑含水量计算及脑组织生化指标的测定来评价8d的抗脑缺血、抗脑水肿和抗氧化作用。结果:与模型组相比,8d在380 mg/kg的剂量下能显著降低脑缺血再灌注损伤大鼠的神经功能学评分、缩小脑梗死面积、减少脑含水量,还具有增加脑组织中谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活力、降低脑组织丙二醛(MDA)和活性氧(reactive oxygen species,ROS)含量的作用;8d 在80 mg/kg剂量时对脑缺血再灌注损伤的保护作用较弱。 结论:8d对大鼠脑缺血再灌注损伤具有明显的保护作用,其机制与抗氧化作用有关。
关 键 词: 老年药理学;8d;脑缺血再灌注损伤;大脑中动脉阻塞;抗氧化
Title: Neuroprotective effects and mechanisms of 3-n-butylphthalide bearing NO/H2S-donating moieties 8d against cerebral ischemia/reperfusion injury in rats
Author: LAN Li, JI Jing, JI Hui
Organization: Department of Pharmacology, China Pharmaceutical University
Abstract: Objective: To determine the potential effects of orally administered 3-n-butylphthalide bearing NO/H2S-donating moieties 8d on rats subjected to ischemia and reperfusion. Methods: With the exception of animals in sham group, all animals in treatment groups were subjected to ischemia/reperfusion by middle cerebral artery occlusion (MCAO) with thread technique. The ability of anticerebral ischemia, relieving cerebral edema and antioxidation was evaluated by the measurement of neurological deficit score, ratios of infarction area, brain water content and brain tissu biochemical indexes related to oxidative stress in brain tissue. Results: Compared with the model group, 8d (380 mg/kg) can significantly reduce the neurological function, infarct size, brain water content. It also increase the level of GSH, GSH-Px, SOD and reduce the contents of MDA and reactive oxygen species (ROS). 8d (80 mg/kg) has a weak protective effect against cerebralischemia. Conclusion: 8d exhibited obvious protection against cerebral ischemia/reperfusion injury due to its antioxidating effect.
Key words: elderly pharmacology; 8d; cerebral ischemia/reperfusion injury; middle cerebral artery occlusion reperfusion; antioxidation
发表期数: 2016年9月第17期
引用格式: 兰 丽,吉 敬,季 晖. NO/H2S双供体型丁苯酞衍生物8d对大鼠脑缺血再灌注损伤的保护作用及其机制[J]. 中国科技论文在线精品论文,2016,9(17):1820-1826.
 
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