您的位置:首页  > 论文页面

莓实假单胞菌双组分信号转导系统的分析

发表时间:2016-11-15  浏览量:2260  下载量:481
全部作者: 徐湘婷,梅艳珍
作者单位: 南京师范大学生命科学学院
摘 要: 莓实假单胞菌是从北极极端环境中分离到的一株耐冷、耐盐细菌。为研究其对极端环境的适应性,基于该菌的基因组测序数据,预测并分析了其双组分信号转导系统(two-component signal transduction system,TCS)的分布、结构及功能。研究鉴定了13对成对TCSs和2个孤儿组氨酸蛋白激酶(histidine protein kinases,HK),发现大多数HK具有接受信号的跨膜区及HAMP、HATPase_c、HisKA等结构域。TCSs主要是OmpR 亚家族,占总TCSs 的61.5%以上;聚类分析显示TCS族、2组孤儿HK存在较大的差异,不同类别的HK分别在不同的进化分支上。莓实假单胞菌株TCS功能涉及氮素固定及代谢调节、重金属响应、铜抗性调控等,这可能有助于菌株在北极极端环境中生存。本研究结果可为莓实假单胞菌在极端环境中的适应机制研究奠定理论基础。
关 键 词: 微生物学;莓实假单胞菌;双组分信号转导系统;组氨酸蛋白激酶;进化分析
Title: Analysis on two-component signal transduction system of Pseudomonas fragi
Author: XU Xiangting, MEI Yanzhen
Organization: College of Life Science, Nanjing Normal University
Abstract: Pseudomonas fragi was isolated from the extreme environment of Arctic with cold tolerance and salt tolerance. The two-component signal transduction system (TCS) of P. fragi was predicted and analyzed based on the whole genome sequencing. 13 pairs of TCSs and 2 orphan histidine protein kinases (HK) were identified, and the majority of HK was found to have the HAMP, HATPase_c, HisKA, and other structural domains. Most of TCSs belong to OmpR sub family, accounted for more than 61.5% of all TCSs. The clustering analysis showed that the HisKAs located in the different evolutionary branch, suggesting the TCSs are diverse. The functions of TCSs in P. fragi involved in nitrogen fixation and metabolism, heavy metals, copper resistance response regulation, and so on. This may contribute to the strain to survive in the Arctic environment. These results will lay a theoretical foundation for the cold tolerance and salt tolerance of P. fragi in extreme conditions.
Key words: microbiology; Pseudomonas fragi; two-component signal transduction system; histidine protein kinase; phylogenetic analysis
发表期数: 2016年11月第21期
引用格式: 徐湘婷,梅艳珍. 莓实假单胞菌双组分信号转导系统的分析[J]. 中国科技论文在线精品论文,2016,9(21):2174-2178.
 
1 评论数 0
暂无评论
友情链接