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拟南芥小GTP结合蛋白RABE1C参与植物干旱胁迫调控研究

发表时间:2016-11-15  浏览量:1777  下载量:349
全部作者: 林敏燕,李春龙,曹花爱,张 伟
作者单位: 山东大学生命科学学院,植物细胞工程与种质创新教育部重点实验室;2. 山东安丘经济开发区管理委员会
摘 要: 干旱是限制植物生长发育和生物量积累的主要逆境因子,而发掘和研究植物自身抗旱功能基因,是以分子育种手段获取耐干旱植物新品种,并减轻干旱胁迫对植物危害的重要途径。本研究发现,拟南芥小GTP结合蛋白RABE1C参与了植物干旱胁迫响应调控,其功能缺失突变体rabe1c-1及rabe1c-2对脱落酸(abscisic acid,ABA)调控气孔运动过程不敏感,抗干旱能力降低。通过GUS染色分析发现,RABE1C在保卫细胞中大量表达;另外RABE1C受到外界干旱胁迫和ABA的诱导表达。以上研究结果表明,RABE1C可能作为一个正调控因子,通过调控气孔运动过程参与植物干旱胁迫响应,进而影响植物的抗干旱能力。
关 键 词: 细胞生物学;干旱胁迫;脱落酸;气孔运动;RABE1C
Title: Positive regulation on the plant drought tolerance of Arabidopsis small GTP binding protein RABE1C
Author: LIN Minyan1, LI Chunlong1, CAO huaai2, ZHANG Wei1
Organization: 1. Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University; 2. Anqiu Economic Development Zone Management Committee
Abstract: Drought is one of the most serious stresses in restraining plant growth and development as well as crop yield. To screen and investigate the plant drought responsive genes is an important way to obtain new essential functional genes and molecular mechanisms and to improve plant drought tolerance and make further development of water-use-efficiency agriculture. In this study, we found that Arabidopsis small GTP binding protein RABE1C was involved in plant drought response. Compared with wild type plants, the RABE1C loss-of-function mutants rabelc-1 and rabelc-2 reduced the plant drought tolerance and showed hyposensitivity to abscisic acid (ABA) regulated stomatal movements. GUS staining assay showed that RABE1C was highly expressed in a serial of tissues, especially in guard cells. Moreover, RABE1C transcription level was induced by drought and ABA treatments. All presented data demonstrated that RABE1C may function as a positive regulator in ABA regulated stomatal movements, and thus to enhance the plant drought tolerance.
Key words: cell biology; drought tolerance; abscisic acid; stomatal movements; RABE1C
发表期数: 2016年11月第21期
引用格式: 林敏燕,李春龙,曹花爱,等. 拟南芥小GTP结合蛋白RABE1C参与植物干旱胁迫调控研究[J]. 中国科技论文在线精品论文,2016,9(21):2179-2187.
 
16 评论数 1

:本文的研究内容有很强的应用价值,引用的文献也很充实,实验设计合理,所得结果可信,并且书写规范,条理清楚,是一篇优秀论文。

2017-02-22 10:21:17
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