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线粒体自噬相关蛋白在新生鼠缺氧缺血皮质神经细胞中的表达
发表时间：2017-03-15 浏览量：1873 下载量：473
| 全部作者： | 李明熹，张莉，屈艺，母得志 |
| 作者单位： | 四川大学华西第二医院儿科；四川大学出生缺陷与相关妇儿疾病教育部重点实验室 |
| 摘 要： | 目的：探讨线粒体自噬相关蛋白在缺氧缺血大鼠皮质神经细胞中的表达，了解在缺氧缺血脑损伤（hypoxic-ischemic brain damage，HIBD）动物模型中线粒体自噬情况。方法：60只雄性7日龄新生SD大鼠随机分为假手术组（sham组）、缺氧缺血脑损伤组（HIBD组）、自噬抑制剂干预组（3-MA组）。在缺氧缺血后2，12，24，48，72 h时，提取皮质神经元中的细胞浆蛋白和线粒体蛋白，采用Western blotting检测线粒体自噬相关蛋白的表达。结果：在细胞浆中，HIBD组LC3II/I、PINK1和Parkin的表达明显增高，与sham组相比差异显著（P＜0.01），在手术12 h后表达开始升高，48 h后开始下降，72 h后逐渐恢复正常；线粒体外膜分裂相关蛋白Drp1、Fis1的表达与PINK1、Parkin一致，在12～24 h表达升高，HIBD组各时间点与sham组相比存在明显差异（P＜0.01），同时，线粒体外膜蛋白Tom20与线粒体内膜蛋白Tim23的表达在手术后逐渐降低，12～24 h表达最低，与sham组相比，差异有统计学意义（P＜0.01）；自噬抑制剂干预后，与sham组及HIBD组各时间点相比，PINK1、Parkin、Drp1、Fis1的表达降低，Tom20和Tim23的表达升高，差异均有统计学意义（P＜0.01）。结论：线粒体自噬相关蛋白在HIBD后的皮质神经元线粒体中的表达具有一定的时间规律性，抑制线粒体自噬可能与HIBD的修复存在一定关系。 |
| 关 键 词： | 儿科学；线粒体自噬；缺氧缺血；脑损伤 |
| Title： | Expression of mitophagy related protein in cortical neurons of hypoxic-ischemic newborn rat |
| Author： | LI Mingxi, ZHANG Li, QU Yi, MU Dezhi |
| Organization： | Department of Pediatrics, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University |
| Abstract： | Objective: To investigate the expression of mitophagy related protein in cortical neurons of hypoxic-ischemic newborn rats and to study the situation of mitophagy in the model of hypoxic-ischemic brain damage (HIBD). Methods: Sixty seven-day-old neonatal male SD rats were randomly divided into three groups: sham-operated group (sham), HIBD model group (HIBD), 3-MA injection group (3-MA). After 2, 12, 24, 48, 72 h of hypoxic-ischemic treatment, the protein in cortical neurons was extracted. Western blotting was used to measure the expression of mitophagy related protein. Results: In cytoplasm, compared with the sham group, the expression of LC3II/I, PINK1 and Parkin in HIBD group were significantly increased (P<0.01) after 12 h of hypoxic-ischemic treatment, decreased after 48 h and back to base-line at 72 h. The same tendency can be observed in the expression of mitochondrial outer membrane fission related protein (P<0.01). Simultaneously, the expression of mitochondrial membrane protein, Tom20 and Tim23, were reduced after surgery, which reached the lowest expression after 12-24 h (P<0.01), compared with sham group. After injecting of 3-MA, an inhibitor of mitophagy, the expression of PINK1/Parkin and Drp1/Fis1 were reduced, oppositely, the expression of Tom20/Tim23 were increased (P<0.01), compared with sham group and HIBD group at each time point. Conclusion: The expression of mitophagy related protein in cortical neurons of HIBD is on a regular or periodic basis, which may have some relationship with the protection role from HIBD. |
| Key words： | pediatrics; mitophagy; hypoxic-ischemic; brain damage |
| 发表期数： | 2017年3月第5期 |
| 引用格式： | 李明熹，张莉，屈艺，等. 线粒体自噬相关蛋白在新生鼠缺氧缺血皮质神经细胞中的表达[J]. 中国科技论文在线精品论文，2017，10（5）：509-517. |
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