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脂肪酸受体在LPS诱导急性肺损伤小鼠中表达变化的研究

发表时间:2017-05-15  浏览量:1582  下载量:284
全部作者: 陈莉莉,王绍莹,殷诚聪,杜冰,钱旻,任华
作者单位: 华东师范大学生命科学学院;华东师范大学生命医学研究所,上海市调控生物学重点实验室
摘 要: 利用脂多糖(lipopolysaccharide,LPS)诱导构建急性肺损伤(acute lung injury,ALI)小鼠模型,研究游离脂肪酸受体(free fatty acid receptor,FFAR)家族在ALI小鼠中表达的变化。气管插管给予LPS方式构建ALI小鼠模型,取小鼠肺组织,经HE染色发现肺部结构完整性被破坏,存在炎性细胞浸润现象。荧光实时定量PCR(quantitative real-time PCR,qRT-PCR)检测发现小鼠肺组织中IL-6和TNF-α表达量升高,同时酶联免疫吸附测定(enzyme linked immunsorbent assay,ELISA)检测肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中IL-6和TNF-α的表达量也显著上升,表明ALI小鼠模型构建成功。进一步用逆转录PCR(reverse transcription PCR,RT-PCR)法分析ALI小鼠肺部脂肪酸受体家族表达情况,发现其家族中Gpr84表达量显著提高。构建Gpr84-/-ALI小鼠模型,检测发现小鼠肺部IL-6和TNF-α表达量与野生型ALI小鼠相比下降明显。研究初步发现敲除Gpr84会降低ALI小鼠肺部的炎症反应,为进一步揭示Gpr84在ALI发生发展中的功能与机制奠定了基础。
关 键 词: 细胞免疫学;急性肺损伤;游离脂肪酸受体家族;Gpr84
Title: Expression change of FFARs in LPS-induced ALI mouse model
Author: CHEN Lili, WANG Shaoying, YIN Chengcong, DU Bing, QIAN Min, REN Hua
Organization: School of Life Science, East China Normal University; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, East China Normal University
Abstract: The acute lung injury (ALI) mouse model was induced with lipopolysaccharide (LPS), and the expression of free fatty acid receptors (FFAR) family was detected. LPS was given by tracheal intubation to construct ALI mouse model and the lung tissues were taken out. HE staining results showed that destruction of structural integrity and inflammatory cell infiltration occurred in the lungs of ALI mice. Quantitative real-time PCR (qRT-PCR) results showed that in lung tissues of ALI mice, the expressions of IL-6 and TNF-α were both up-regulated, which was coincident with the enzyme linked immunosorbent assay (ELISA) results of bronchoalveolar lavage fluid (BALF). The results showed that ALI mouse model was successfully constructed. Meanwhile, the expression of FFARs in ALI mice was further analyzed by reverse transcription PCR (RT-PCR). The expression of Gpr84 was significantly increased in ALI mice. To explore whether Gpr84 was involved in the process of ALI, we constructed the LPS-induced ALI in Gpr84-/- mice. The results indicated that in Gpr84-/- ALI mice, the inflammation was relieved and the expressions of IL-6 and TNF- were both down-regulated, compared with wild type (WT). It was found that knockdown of Gpr84 could decrease the inflammatory response in ALI mice, which laid the foundation for further study on the function and mechanism of Gpr84 in the development of ALI.
Key words: cellular immunology; acute lung injury; free fatty acid receptor family; Gpr84
发表期数: 2017年5月第9期
引用格式: 陈莉莉,王绍莹,殷诚聪,等. 脂肪酸受体在LPS诱导急性肺损伤小鼠中表达变化的研究[J]. 中国科技论文在线精品论文,2017,10(9):933-939.
 
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