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香料化合物广谱降解菌的分离、鉴定与降解性能分析

发表时间:2017-05-15  浏览量:1502  下载量:297
全部作者: 刘会会,古静燕,李新,卫洁,韩文君
作者单位: 山东大学国家糖工程技术研究中心;济南悟通生物科技有限公司
摘 要: 从香料厂的污水处理池样品中分离微生物,采用唯一碳源法检测菌株对香料化合物的利用能力,基于16S rRNA基因的克隆与测序进行分子鉴定,监测化学需氧量(chemical oxygen demand,COD)值和氨氮含量的变化,从而分析混合使用广谱降解菌对香料厂污水的处理能力。结果表明,获得5株广谱降解菌分别鉴定至节杆菌属(Arthrobacter sp.)、假单胞菌属(Pseudomonas sp.)、颇陌菌属(Advenella sp.)和变形杆菌属(Proteus sp.),能高效降解利用多种香料化合物,混合菌株处理污水可使COD值降到100 mg/L以下,氨氮含量降到15 mg/L以下,半衰期为5 d,污水达到排放标准。
关 键 词: 微生物学;香料化合物降解菌;16S rRNA基因;降解性能
Title: Isolation, identification and degradation ability of extensive flavor-degrading strains
Author: LIU Huihui, GU Jingyan, LI xin, WEI Jie, HAN Wenjun
Organization: National Glycoengineering Research Center, Shandong University; Jinan Enlighten Biotechnology Co., Ltd.
Abstract: Extensive flavor-degrading strains were isolated from the activated sludge tank of a spice factory. Various flavor products were used as the sole carbon source to screen the flavor-degrading microorganisms. The 16S rRNA genes were cloned, sequenced and analyzed to identify the taxonomic position of strains. The sewage treatment effects were analyzed by monitoring the change of chemical oxygen demand (COD), and ammonia nitrogen contents. As a result, five flavor-degrading strains have been identified as members that belong to the Arthrobacter sp., Pseudomonas sp., Advenella sp., and Proteus sp. genuses respectively. These strains can degrade a variety of flavor compounds and the bacterial mixture can drop COD below 100 mg/L and the ammonia nitrogen below 15 mg/L. The half-life is 5 d. Wastewater discharge can meet up its standard.
Key words: micobiology; flavor-degrading strains; 16S rRNA gene; degradation ability
发表期数: 2017年5月第9期
引用格式: 刘会会,古静燕,李新,等. 香料化合物广谱降解菌的分离、鉴定与降解性能分析[J]. 中国科技论文在线精品论文,2017,10(9):948-953.
 
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