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小麦TaTGA2基因的克隆及赤霉病抗性功能验证

发表时间:2017-05-15  浏览量:1455  下载量:214
全部作者: 苏培森,王彪,尹华燕,王宏伟,孔令让,李安飞
作者单位: 山东农业大学农学院,作物生物学国家重点实验室
摘 要: 在模式植物拟南芥中,TGA2在介导水杨酸(salicylic acid,SA)信号传递响应植物防御中发挥重要作用。通过同源克隆,从小麦抗赤霉病种质苏麦3号中获得全长1 011 bp的TaTGA2 基因,其具有亮氨酸拉链保守结构域,属于bZIP家族的转录因子。过量表达TaTGA2的二穗短柄草对小麦赤霉病表现出较好的抗性;同时利用BSMV-VIGS技术将TaTGA2沉默后,苏麦3号对赤霉病的抗性减弱。另外,亚细胞定位结果表明,该基因被定位在细胞核。实时定量PCR(quantitative real-time PCR,qRT-PCR)结果显示,TaTGA2响应SA信号的调控表达并且明显上调。因此,小麦转录因子TaTGA2可能在抗赤霉病过程中发挥重要作用。
关 键 词: 作物遗传学;小麦;赤霉病;TaTGA2
Title: Cloning of TaTGA2 and Function Identification to Fusarium head blight(FHB) in Wheat
Author: SU Peisen, WANG Biao, YIN Huayan, WANG Hongwei, KONG Lingrang, LI Anfei
Organization: State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University
Abstract: In the model plant Arabidopsis thaliana, TGA2 plays an important role in plant defense response by mediating salicylic acid (SA) signaling. We obtained a full-length 1 011 bp TaTGA2 gene from Wheat Scab Resistant Germplasm Sumai No.3 by homologous cloning, which is a transcription factor belongs to the bZIP family and has the leucine zipper domain. Overexpression of TaTGA2 in Brachypodium distachyon showed better resistance to Fusarium graminearum infection. BSMV-VIGS TaTGA2 silencing in Sumai No.3 led to increased susceptibility to F. graminearum infection compared with controls. In addition, we carried out subcellular localization and located TaTGA2 in the nucleus. Quantitative real-time PCR (qRT-PCR) assay revealed that the TaTGA2 gene could be induced by SA and up-regulated significantly, which suggests that TaTGA2 gene plays an important role in the defense response to F. graminearum.
Key words: crop genetics; wheat; Fusarium head blight; TaTGA2
发表期数: 2017年5月第9期
引用格式: 苏培森,王彪,尹华燕,等. 小麦TaTGA2基因的克隆及赤霉病抗性功能验证[J]. 中国科技论文在线精品论文,2017,10(9):979-988.
 
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