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草莓冷响应转录因子FaICE1的克隆及其互作蛋白分析

发表时间:2017-05-15  浏览量:1554  下载量:193
全部作者: 魏灵芝,贾美茹,杜平,李基正,贾文锁,李冰冰
作者单位: 中国农业大学园艺学院
摘 要: 低温是影响冬季温室草莓生产的重要因素之一,克隆并鉴定抗冷相关基因对草莓理论研究和实际生产均有重要意义。利用八倍体草莓(Fragaria × ananassa)品种‘红颜’作为试验材料,克隆并鉴定了一个低温响应的转录因子——FaICE1. 基因表达分析结果显示,随着发育进程的推进,在果实发育前期FaICE1的表达呈现下降的趋势,白果期后其表达趋于稳定。同时,果实圆片试验表明低温胁迫(4℃)和茉莉酸(jasmonic acid,JA)均能够诱导FaICE1的表达。双分子荧光互补(bimolecular fluorescence complementation,BiFC)试验进一步证明,FaICE1与草莓果实抗低温关键信号蛋白FaSnRK2.6存在直接蛋白互作,暗示FaICE1可能是FaSnRK2.6的下游信号组分,参与了低温条件下草莓果实发育和成熟的调控。
关 键 词: 果树学;草莓;低温;FaICE1;FaSnRK2.6;果实发育和成熟
Title: Cloning of cold response transcription factor FaICE1 from strawberry and analysis of its interacting proteins
Author: WEI Lingzhi, JIA Meiru, DU Ping, LI Jizheng, JIA Wensuo, LI Bingbing
Organization: College of Horticulture, China Agricultural University
Abstract: Low temperature is one of the important factors affecting strawberry production in winter greenhouse. Cloning and identification of the genes related to cold resistance are of great significance for theoretical research and practical production of strawberry. A transcription factor, FaICE1, in response to low temperature was cloned and identified in octoploid strawberry (Fragaria × ananassa) named ‘Hongyan’. The expression of FaICE1 gene decreased at the early stage of fruit development, and was stable after the stage of white fruit. Fruit wafer test showed that the expression of FaICE1 could be induced by low temperature (4℃) stress and jasmonic acid (JA), respectively. Bimolecular fluorescence complementation (BiFC) further proved that FaICE1 interacted directly with FaSnRK2.6, which is the key signal protein of strawberry fruit resistance to low temperature, suggesting that FaICE1 might be the downstream signal component of FaSnRK2.6 and participated in the development and ripening regulation of strawberry fruit under low temperature stress.
Key words: pomology; strawberry; low temperature; FaICE1; FaSnRK2.6; fruit development and ripening
发表期数: 2017年5月第9期
引用格式: 魏灵芝,贾美茹,杜平,等. 草莓冷响应转录因子FaICE1的克隆及其互作蛋白分析[J]. 中国科技论文在线精品论文,2017,10(9):989-995.
 
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