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BMP11基因在A549细胞中放射增敏作用的研究

发表时间:2017-06-15  浏览量:88  下载量:29
全部作者: 赵晓岚,李素素,周新文
作者单位: 苏州大学医学部放射医学与防护学院
摘 要: 目的:探讨骨形态发生蛋白11(bone morphogenetic proteins 11,BMP11)基因高表达对人非小细胞肺癌A549细胞放射敏感性的影响。方法:Western blotting法检测肺常见细胞中BMP11基因和蛋白的表达。采用pcDNA3.1构建BMP11基因表达载体。以人非小细胞肺癌A549细胞为主要研究对象,设BMP11转染组(A549/BMP11)、pcDNA3.1转染组(A549/NC)和空白对照组(A549)。克隆形成实验检测细胞的放射敏感性,噻唑蓝(MTT)实验检测细胞的生长和增殖,流式细胞仪检测其凋亡变化。结果:检测细胞均表达BMP11蛋白,但表达含量各不相同,且BMP11参与了放射敏感性的调节;克隆形成实验显示,BMP11转染组与对照组相比,克隆形成能力明显下降,联合处理组与照射组相比,其放射敏感性增加;MTT实验显示,BMP11明显抑制了细胞的生长;流式细胞仪显示,BMP11转染组的凋亡率明显高于对照组,P<0.05. 结论:BMP11参与了放射敏感性的调节,且可通过诱导细胞凋亡抑制A549细胞的增殖,BMP11基因的高表达能够增加A549细胞的放射敏感性,其机制可能与诱导细胞凋亡有关。
关 键 词: 放射医学;放射敏感性;BMP11;A549;细胞凋亡
Title: Study on radiosensitivity of BMP11 on A549 cell
Author: ZHAO Xiaolan, LI Susu, ZHOU Xinwen
Organization: School of Radiation Medicine and Protection, Medical College of Soochow University
Abstract: Objective: To explore the role of bone morphogenetic proteins 11 (BMP11) overexpression in the radiosensitivity of human non-small cell lung cancer A549 cell. Methods: The expression of BMP11 gene and protein was detected by Western blotting respectively. BMP11 gene expression vector was constructed by pcDNA3.1, and then was transferred into human non-small lung cancer A549 cells with lipofectamine. The experiments were divided into A549/BMP11 group, A549/NC group and blank control group. Radiosensitivity was evaluated by colony formation assay. The cell growth and proliferation were detected by using MTT kit. Flow cytometry was applied to analyze apoptosis. Results: BMP11 protein was expressed in cells, but the expression levels were different, and were involved in the regulation of radiosensitivity. Colony forming assay showed that the colony forming ability of the transfected group was significantly decreased compared with the control group, and the radiosensitivity of the combined treatment group was increased compared with the radiation group. MTT experiments showed that BMP11 significantly inhibited the growth of cells. Flow cytometry showed that the apoptosis rate of BMP11 transfected group was significantly higher than control group, P<0.05. Conclusion: BMP11 is involved in the regulation of radiosensitivity, and can inhibit the increase of A549 cell by inducing apoptosis. BMP11 gene overexpression can increase the radiosensitivity of A549 cell, and its mechanism may be related to the induction of apoptosis.
Key words: radioactive medicine; radiosensitivity; BMP11; A549; apoptosis
发表期数: 2017年6月第11期
引用格式: 赵晓岚,李素素,周新文. BMP11基因在A549细胞中放射增敏作用的研究[J]. 中国科技论文在线精品论文,2017,10(11):1261-1267.
 
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