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胶体金检测卡光电检测关键技术研究

发表时间:2017-06-15  浏览量:95  下载量:8
全部作者: 费晚茹,侯玲玲,尹树雨,齐元,程成,陶晓杰,宫平
作者单位: 长春理工大学生命科学技术学院
摘 要: 为提高胶体金检测的精准性,减少观察者主观因素导致检测结果出现假阴性、假阳性的现象,提出一种光电检测的方法对胶体金检测卡检测信息进行半定量分析。利用滨松S1133进行信号采集,采用STM32F429作为核心处理单元,结合检测线位置标定技术,建立检测仪器与胶体金检测卡封闭的半定量检测方法。与高效液相色谱(high performance liquid chromatography,HPLC)进行方法学比对,对仪器进行定标,使检测样本中抗原浓度与检测信号相对应,实现对胶体金检测卡高灵敏度的快速检测。通过实验分析,对三聚氰胺检测的准确度达±12%,对盐酸克伦特罗、莱克多巴胺和沙丁胺醇的最低检测限分别为3,5,10 ng/mL. 采用光电检测的方法可以提高胶体金检测技术的精准性和检测水平,非常适合现场快速筛查。
关 键 词: 生物医学工程学;测试计量技术及仪器;光电检测;胶体金
Title: Research on key technology of photoelectric detection on colloidal gold detection card
Author: FEI Wanru, HOU Lingling, YIN Shuyu, QI Yuan, CHENG Cheng, TAO Xiaojie, GONG Ping
Organization: School of Life Science and Technology, Changchun University of Science and Technology
Abstract: In order to improve the accuracy of colloidal gold detection, and reduce the subjective factors of the observer which leads to false negative and false positive results, a photoelectric detection method is proposed to semiquantitatively analyze the information of colloidal gold detection card. Hamamatsu S1133 is used for signal acquisition, and STM32F429 is used as the core processing unit. Combined with the position detection technology, a closed semiquantitative detection method of detected instrument and colloidal gold detection card are established. Compared with high performance liquid chromatography (HPLC) method, corresponding concentration of antigen in the sample and the detection signal should be achieved by calibrating the instrument, then rapid detection of colloidal gold detection card for high sensitivity is achieved. Through the experimental analysis, the accuracy of melamine detection can reach to ±12%, the lowest detection limits of clenbuterol, ractopamine and salbutamol are 3, 5, 10 ng/mL, respectively. Accuracy and detection level of colloidal gold detection are improved by using the method of photoelectric detection, which is suitable for rapid screening in the field.
Key words: biomedical engineering; measurement technology and instruments; photoelectric detection; colloidal gold
发表期数: 2017年6月第11期
引用格式: 费晚茹,侯玲玲,尹树雨,等. 胶体金检测卡光电检测关键技术研究[J]. 中国科技论文在线精品论文,2017,10(11):1276-1280.
 
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