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不同时间点胎肝proB发育及分化B1a能力研究

发表时间:2017-08-15  浏览量:42  下载量:8
全部作者: 杨智森,刘清枝,廉哲雄
作者单位: 中国科学技术大学生命科学学院
摘 要: 目的:研究小鼠不同胎龄proB细胞发育及分化B1a能力的差异。方法:获取不同胎龄的野生型小鼠胎肝(E7、E12、E15),分离淋巴细胞,运用流式细胞仪检测proB水平并分析其变化趋势。运用流式细胞分选仪分选E12胎肝proB细胞,转输至半致死剂量辐照的受体鼠Rag1/,转输6周后牺牲小鼠研究其分化腹腔B1a的能力。结果:野生型小鼠E7胎肝中没有检测到B细胞,E12和E15的胎肝中均出现了B细胞和proB细胞。数据统计分析表明,随着胎龄增加,胎肝中B(E12 vs E7:P=0.004 1;E15 vs E12:P=0.000 3)和proB(E12 vs E7:P=0.027 1;E15 vs E12:P=0.108 8)细胞的比例逐渐上升。转输结果表明,E12胎肝proB细胞很好地重建了B1a细胞。结论:野生型小鼠随着胎龄的增加,胎肝中B细胞前体proB细胞出现且比例逐渐增加,具有重建腹腔B1a细胞的能力。
关 键 词: 细胞生物学;发育;胎肝;proB细胞;B1a细胞
Title: Development and differentiation of fetal liver proB cell into B1a cell on different embryonic days
Author: YANG Zhisen, LIU Qingzhi, LIAN Zhexiong
Organization: School of Life Sciences, University of Science and Technology of China
Abstract: Objective: To study the development and differentiation of fetal liver proB cells into Bla cell from various embryonic day (E) of wild type mice. Methods: We obtained E7, E12, E15 fetal liver of wild type mice and used the lymphocyte separation flow cytometry for proB cells level detection and their variation tendency analysis. And we also sorted proB cells from E12 fetal liver and transferred proB cells into medial lethal doses of 3 Gy irradiated Rag1/ mice intravenously. 6 weeks later, we sacrificed the recipient mice to detect peritoneal cavity B1a cells. Results: There were no B cells detected in E7 fetal liver of wild type mice. But B and proB cells could be detected in E12 and E15 fetal liver and the percentages of B (P=0.004 1 for E12 vs E7, P=0.000 3 for E15 vs E12) and proB (P=0.027 1 for E12 vs E7, P=0.108 8 for E15 vs E12) cells were increased with age. The adoptive transfer experiment showed that proB cells from E12 fetal liver could rebuild B1a cells well. Conclusion: proB cells, a precursor of B cells, appeared in fetal liver at medium gestational mouse, increased with age and differentiated into peritoneal cavity B1a cells.
Key words: cell biology; development; fetal liver; proB cell; Bla cell
发表期数: 2017年8月第15期
引用格式: 杨智森,刘清枝,廉哲雄. 不同时间点胎肝proB发育及分化B1a能力研究[J]. 中国科技论文在线精品论文,2017,10(15):1688-1693.
 
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