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异养硝化-好氧反硝化菌Bacillus sp. JB4的异养硝化特性研究

发表时间:2017-08-15  浏览量:1744  下载量:188
全部作者: 李泳洪,陈小岚,许旭萍
作者单位: 福建师范大学生命科学学院
摘 要: 为进一步探究已筛选并分离纯化出的异养硝化-好氧反硝化菌JB4(Bacillus sp. JB4)的异养硝化活性,采用单因素对照实验,并结合相关分光光度法,分别研究pH值、温度、接种量、转速、碳源、底物浓度和C/N等因素对该株异养硝化-好氧反硝化菌去除氨氮的影响。结果显示该菌株适宜的脱氮条件为:初始氨氮浓度50~75 mg/L,碳源为丁二酸钠,C/N为8,温度35℃,转速180 r/min,接种量3%,pH值为7.0. 在此条件下,12 h内氨氮去除率为76.95%~83.54%,表明菌株JB4具有良好的异养硝化能力。
关 键 词: 微生物学;异养硝化;单因素对照;芽孢杆菌;生物脱氮
Title: Heterotrophic nitrification characteristics of heterotrophic nitrification-aerobic denitrification strain Bacillus sp. JB4
Author: LI Yonghong, CHEN Xiaolan, XU Xuping
Organization: College of Life Sciences, Fujian Normal University
Abstract: The activity of heterotrophic nitrificationis explored in this study, which has been screened and isolated from the heterotrophic nitrification-aerobic denitrification bacteria JB4 (Bacillus sp. JB4). The effect of pH, temperature, inoculation amount, shaking speed, carbon source, substrate concentration and C/N on the heterotrophic nitrification-aerobic denitrifying bacteria for ammonia nitrogenremoval were researched by using single factor control experiment, combined with spectrophotometry respectively. The results show that the appropriate nitrogen removal conditions are as follows: initial ammonia nitrogen concentration is 50-75 mg/L, sodium succinate is the best carbon source, C/N is 8, temperature is 35℃, shaking speed is 180 r/min, inoculation quantity is 3%, and pH value is 7.0. Strain JB4 shows good heterotrophic nitrification ability under above conditions, and the removal rate of ammonia nitrogen is 76.95%-83.54% within 12 h.
Key words: microbiology; heterotrophic nitrification; single factor control; Bacillus sp.; biological nitrogen removal
发表期数: 2017年8月第15期
引用格式: 李泳洪,陈小岚,许旭萍. 异养硝化-好氧反硝化菌Bacillus sp. JB4的异养硝化特性研究[J]. 中国科技论文在线精品论文,2017,10(15):1694-1700.
 
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