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噬菌体展示筛选与狂犬病毒P互作的宿主蛋白

发表时间:2017-08-15  浏览量:31  下载量:16
全部作者: 董婉玉,李有文,傅振芳,彭贵青
作者单位: 华中农业大学动物医学院,农业微生物学国家重点实验室
摘 要: 首先表达纯化了狂犬病毒(rabies virus,RABV)P蛋白,以纯化的P蛋白为靶蛋白,通过T7噬菌体展示的方法筛选相互作用蛋白。通过5轮的筛选,得到多个阳性克隆,其中核糖体大亚基蛋白L9(ribosomal protein L9,RPL9)与P蛋白特异性相互作用,并通过酶联免疫吸附测定(enzyme-linked immuno sorbent assay,ELISA)、GST pull-down得到验证,为进一步阐明RABV的复制和致病机理奠定基础。
关 键 词: 家畜病毒学;狂犬病毒;P蛋白;噬菌体展示技术;相互作用
Title: Screening of the host proteins interacting with rabies virus P by phage display system
Author: DONG Wanyu, LI Youwen, FU Zhenfang, PENG Guiqing
Organization: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Abstract: The study firstly expressed and purified the rabies virus (RABV) P protein, and we used the purified P protein as the target to screen the interaction protein by a T7 phage display system. Through 5 rounds of elutriation, we obtained several positive clones. The results showed that ribosomal protein L9 (RPL9) could interacted with P protein specifically, which was demonstrated by enzyme-linked immuno sorbent assay (ELISA) and GST pull-down. The study laid the foundation to clarify the replication and pathogenesis of RABV further.
Key words: livestock virology; rabies virus; P protein; phage display technology; interaction
发表期数: 2017年8月第15期
引用格式: 董婉玉,李有文,傅振芳,等. 噬菌体展示筛选与狂犬病毒P互作的宿主蛋白[J]. 中国科技论文在线精品论文,2017,10(15):1722-1728.
 
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