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微囊藻毒素对斑马鱼食欲调控基因的影响

发表时间:2017-08-15  浏览量:25  下载量:9
全部作者: 李广宇,李莉,方洁,钟兆山,薛理耀,何焱
作者单位: 华中农业大学水产学院
摘 要: 蓝藻水华大规模爆发时会产生大量有毒的微囊藻毒素(microcystin,MC),具有极大的危害。通过实验研究MC-LR对斑马鱼食欲的影响,选取不同剂量的MC-LR加入各组水体中,其中实验组的MC-LR终浓度依次为0.3,3,30 μg/L,对照组的MC-LR浓度为0. MC-LR暴露12周后采样,通过取样提取斑马鱼脑RNA,反转录为cDNA,利用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)检测食欲相关基因的转录变化。实验结果表明,当MC-LR浓度较低时,促食欲基因表达降低,MC-LR浓度升高后,促食欲基因表达升高,食欲会旺盛;同时也检测到,在MC-LR的存在下,抑制食欲基因表达降低,随后升高达到一个较稳定的状态。可见,MC-LR可通过干扰食欲相关基因影响鱼类的摄食。
关 键 词: 水产学基础学科;微囊藻毒素;斑马鱼;促食欲基因;抑制食欲基因
Title: Effect of MC-LR on appetite-regulating genes in zebrafish
Author: LI Guangyu, LI Li, FANG Jie, ZHONG Zhaoshan, XUE Liyao, HE Yan
Organization: College of Fisheries, Huazhong Agricultural University
Abstract: A large amount of microcystin (MC) would appear when large-scale blue-green algae breaks out, which has strong damages to environment. In this study, zebrafish were exposed to varied doses of MC-LR groups (0, 0.3, 3, 30 μg/L). After 12 weeks, RNA was extracted from the brain samples and reverse transcribed into cDNA. Fluorescence quantitative real-time PCR (qRT-PCR) reactions were performed to detect the expression of appetite-related genes and assess the changes of zebrafish appetite. The results showed that the expression of appetite-promoting genes decreased at a lower dose MC-LR group, while the expression of appetite-promoting genes increased at a higher dose MC-LR group. In addition, MC-LR could down-regulate the expression of appetite-inhibiting genes at a lower concentration, but it would up-regulate them in a higher concentration and reach to a stable state. MC-LR could disorder appetite-related genes, which might eventually interfere ingestion of zebrafish.
Key words: basic subject of aquatic science; microcystin; zebrafish; appetite-promoting genes; appetite-inhibiting genes
发表期数: 2017年8月第15期
引用格式: 李广宇,李莉,方洁,等. 微囊藻毒素对斑马鱼食欲调控基因的影响[J]. 中国科技论文在线精品论文,2017,10(15):1738-1743.
 
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