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有氧运动对肥胖小鼠胃组织细胞凋亡的相关研究

发表时间:2017-08-15  浏览量:96  下载量:10
全部作者: 龚丽景,魏昊,朱镕鑫,武薇,胡扬
作者单位: 北京体育大学科学研究中心;北京体育大学运动人体科学学院
摘 要: 目的:探讨有氧运动对肥胖小鼠胃组织细胞凋亡相关蛋白水平的影响。方法:40只C57BL/6J小鼠随机分为普通膳食组(NC组)8只和高脂饮食组(H组)32只;建立肥胖模型后,再将高脂饮食组随机分为肥胖对照组(HC组)和肥胖运动组(HE组)。HE组进行4周有氧运动干预,最后测定所有组小鼠血清中的葡萄糖(glucose,GLU)、甘油三酯(triglyceride,TG)和胆固醇(cholesterol,CHO)水平,观察胃组织的形态变化和细胞凋亡情况,记录胃组织中Caspase-3、Caspase-8、Caspase-9的mRNA水平,以及Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2的相对蛋白表达水平。结果:1)高脂饲料饲养后的小鼠血清GLU、CHO水平上升,TG水平下降;运动干预后,小鼠血清GLU水平下降,CHO保持高水平。2)HC组小鼠胃组织排列不规律,组织间缝隙增宽;HE组较HC组组织间缝隙增宽的现象有所改善,但排列仍不够致密。3)HC组小鼠胃组织凋亡率远高于NC组小鼠,而HE组细胞凋亡数量基本恢复到NC组水平。4)与NC组相比,HC组胃组织Caspase-3、Caspase-8、Caspase-9的mRNA水平高,Caspase-9的蛋白表达水平较低;HE组Caspase-3、Caspase-9的mRNA与蛋白相比HC组表达较少,Caspase-8的mRNA表达水平升高;HC组Bax/Bcl-2>1,HE组Bax/Bcl-2≈1. 结论:有氧运动可改善高脂饮食引发的肥胖小鼠胃组织细胞凋亡,减少肥胖对机体胃部组织造成的损伤。
关 键 词: 运动生理学;细胞凋亡;肥胖;胃组织;有氧运动
Title: Study on the apoptosis of gastric tissue in obese mice induced by aerobic exercise
Author: GONG Lijing, WEI Hao, ZHU Rongxin, WU Wei, HU Yang
Organization: Sport Science Research Center, Beijing Sport University;Sport Science College, Beijing Sport University
Abstract: Objective: To explore the impact of aerobic exercise on apoptosis proteins level of gastric tissues of obese mice. Methods: 40 C57BL/6J mice were randomly divided into normal diet group (group NC, n=8) and high-fat diet group (group H, n=32). After the obesity model established, group H was randomly divided into high-fat obesity control group (group HC) and high-fat exercise group (group HE). The level of glucose (GLU), triglyceride (TG) and cholesterol (CHO) of all mice serum were measured, the morphological changes in gastric tissues and apoptosis level were observed by HE staining and TUNEL, the level of Caspase-3, Caspase-8, Caspase-9 mRNA, the relative protein expression level of Caspase-3, Caspase-8, Caspase-9, Bax and Bcl-2 in gastric tissues were detected after group HE 4 weeks aerobic training. Results: 1) Compared with NC, GLU and CHO in mice serum increased and TG decreased in HC mice, but GLU decreased in HE, CHO kept high in serum. 2) Gastric tissues of mice were irregular arrangement in group HC, the gap between tissues was wider than group NC, and the phenomenon of tissue gap in group HE had improved than that in group HC, however the arrangement was still not tight enough. 3) The apoptotic rate in group HC was much higher than group NC, and the apoptotic rate in group HE was back to the level of group NC. 4) Compared to group NC, Caspase-3, Caspase-8 and Caspase-9 mRNA in group HC gastric tissues were higher, but protein expression of Caspase-9 was relatively lower. mRNA and protein expressions of Caspase-3 and Caspase-9 in group HE were both lower than group HC, and Caspase-8 mRNA expression was higher than HC. The rate of protein expression of Bax/Bcl-2 in group HC was more than 1, and recovered to 1 in group HE. Conclusion: The apoptosis of obese mice gastric tissues could be improved by aerobic exercise, which could help to reduce the damage of gastric tissues of organism caused by high-fat diet.
Key words: sports physiology; apoptosis; obesity; gastric tissue; aerobic exercise
发表期数: 2017年8月第15期
引用格式: 龚丽景,魏昊,朱镕鑫,等. 有氧运动对肥胖小鼠胃组织细胞凋亡的相关研究[J]. 中国科技论文在线精品论文,2017,10(15):1772-1779.
 
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