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表达PyMSP9蛋白的重组腺病毒疫苗构建及其免疫原性检测

发表时间:2017-12-15  浏览量:840  下载量:108
全部作者: 张艳晖,吴赐鸿,刘光英
作者单位: 福建医科大学消化道恶性肿瘤教育部重点实验室
摘 要: 疟原虫裂殖子表面蛋白9(merozoite surface protein 9,MSP9)是一个潜在的疟疾疫苗候选抗原,但目前对于MSP9的全长蛋白的免疫原性研究还未见报道。利用人5型腺病毒载体构建表达约氏疟原虫裂殖子表面蛋白9(Plasmodium yoelii merozoite surface protein 9,PyMSP9)的重组腺病毒疫苗,研究该疫苗在小鼠体内的免疫原性。结果显示,成功构建了表达PyMSP9蛋白的重组腺病毒疫苗,并且将该疫苗免疫BALB/c小鼠,免疫后获得的小鼠免疫血清能有效识别重组蛋白PyMSP9-Nt片段。该结果表明,表达PyMSP9蛋白的重组腺病毒疫苗能有效激活小鼠的免疫反应,具有一定的免疫原性,为MSP9疟疾疫苗的开发提供了理论基础。
关 键 词: 医学寄生虫学;疟原虫;裂殖子表面蛋白9;腺病毒疫苗;免疫原性
Title: Construction of human adenoviral recombinant vaccine expressing Plasmodium yoelii merozoite surface protein 9 and its immunogenicity detection
Author: ZHANG Yanhui, WU Cihong, LIU Guangying
Organization: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Fujian Medical University
Abstract: Malaria parasite merozoite surface protein 9 (MSP9) is one of the malaria vaccine candidate, but the immunogenicity study of full MSP9 protein is not reported at present. In this paper, human adenoviral recombinant vaccine expressing Plasmodium yoelii merozite surface protein 9 (PyMSP9) is constructed from human type 5 adenoviruse vector, and its immunogenicity is detected in mice. The result shows that the human adenoviral recombinant vector expressing PyMSP9 is successfully prepared and used for BALB/c mice immunization, and then the mice immune serum could recognize the recombinant PyMSP9-Nt protein. It indicates that human adenoviral recombinant vector expressing PyMSP9 can effectively induce the mice immune response, and its immunogenicity is successfully detected. It provides the theoretical basis for the development of MSP9 vaccine.
Key words: medical parasitology; Plasmodium; merozoite surface protein 9; human adenoviral vaccine; immunogenicity
发表期数: 2017年12月第23期
引用格式: 张艳晖,吴赐鸿,刘光英. 表达PyMSP9蛋白的重组腺病毒疫苗构建及其免疫原性检测[J]. 中国科技论文在线精品论文,2017,10(23):2613-2619.
 
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