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预先低剂量微波辐射增强γ射线诱导小鼠白血病L1210细胞凋亡
发表时间：2019-03-22 浏览量：1489 下载量：134
| 全部作者： | 高振，谢文，樊彩云，曹毅 |
| 作者单位： | 苏州大学医学部公共卫生学院 |
| 摘 要： | 目的：研究预先低剂量微波辐射对γ射线诱导L1210细胞凋亡的影响。方法：将小鼠白血病L1210细胞随机分为对照组（C）、单纯微波组（RF）、单纯γ射线组（γ）和联合暴露组（RF+γ）。单纯微波组和联合暴露组给予120 μW/cm2微波辐射，4 h/d，连续5 d；第5天给予单纯γ射线组和联合暴露组8 Gy γ射线照射。用钙离子依赖性磷脂结合蛋白-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双标记方法检测细胞凋亡，通过Western blotting检测Survivin蛋白的表达水平。结果：与对照组相比，单纯γ射线组凋亡率显著升高（P<0.05）；与单纯γ射线组相比，联合暴露组凋亡率显著升高（P<0.05）。与对照组相比，单纯γ射线组细胞内Survivin蛋白表达水平显著降低（P<0.05）；与单纯γ射线组相比，联合暴露组Survivin蛋白表达水平显著降低（P<0.05）。结论：在本实验条件下，8 Gy γ射线可以引起小鼠白血病L1210细胞凋亡率升高，Survivin蛋白表达水平降低。预先900 MHz、120 μW/cm2微波辐射能够显著增强γ射线引起的小鼠白血病L1210细胞的损伤，表现为细胞凋亡率升高和Survivin蛋白表达水平降低。 |
| 关 键 词： | 毒理学；凋亡；低剂量微波；γ射线 |
| Title： | Apoptosis of mouse leukemia L1210 cells induced by pre-low-dose microwave radiation-enhanced γ-rays |
| Author： | GAO Zhen, XIE Wen, FAN Caiyun, CAO Yi |
| Organization： | School of Public Health, Medical College of Soochow University |
| Abstract： | Objective: To investigate the role of pre-low-dose microwave radiation on γ-rays inducing the apoptosis of L1210 cell. Methods: L1210 cells were radomly divided into control group (C), microwave alone group (RF), γ-rays alone group (γ) and combined exposure group (RF+γ). Microwave alone group and combined exposure group were exposed to 120 μW/cm2 microwave, 4 h/d, for 5 days. The combined exposure group and γ-rays alone group were subsequently exposed to 8 Gy γ-rays on 5th day. Cell apoptosis was detected with Annexin V-FITC/PI double labelling method. Survivin protein expression level was measured with Western blotting. Results: Compared with control group, the apoptosis rate of cells in γ-rays alone group significantly increased (P<0.05). The apoptisis rate of cells in combined exposure group was statistically higher than that of γ-rays alone group (P<0.05). Compared with control group, Surviving protein expression level of γ-rays alone group decreased significally (P<0.05). Survivn protein expression level of combined exposure group was statistically lower than that of γ-rays alone group (P<0.05). Conclusion: Under this experimental condition, 8 Gy γ-rays could increase the apoptosis rate and decreased Survivin protein expression level in L1210 cells. Pre-exposure with 900 MHz, 120 μW/cm2 microwave radiation could significantly increase the γ-rays induced damages to L1210 cells, as evidenced by the increase of apoptosis rate and decrease of Survivin protein expression level. |
| Key words： | toxicology; apoptosis; low-dose microwave radiation; γ-rays |
| 发表期数： | 2019年2月第1期 |
| 引用格式： | 高振，谢文，樊彩云，等. 预先低剂量微波辐射增强γ射线诱导小鼠白血病L1210细胞凋亡[J]. 中国科技论文在线精品论文，2019，12（1）：156-161. |
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