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介孔硅纳米粒的聚多巴胺修饰及药物负载的研究

发表时间:2021-09-30  浏览量:81  下载量:9
全部作者: 王璐,张睿,邓益斌,陶婧
作者单位: 苏州大学药学院;苏州大学附属第一医院
摘 要: 目的:介孔硅纳米粒(mesoporous silica nanoparticles,MSNs)经聚多巴胺(polydopamine,PDA)修饰后,负载化疗药物阿霉素(doxorubicin,DOX)以及光热材料Cypate,制得载Cypate/DOX介孔硅纳米粒。方法:本研究以十六烷基三甲基氯化铵(CTAC)为模板,在碱性条件下使硅源正硅酸四乙酯(TEOS)水解聚合形成表面带有羟基的介孔硅(MSN-OH),去除表面活性剂后,得到介孔硅纳米粒。经PDA修饰后,采用4-羟乙基哌嗪乙磺酸(HEPES)缓冲液法制备得到MSN-NH2-PDA,采用三乙胺脱盐法制备得到载DOX/Cypate介孔硅纳米粒(MSN-PDA-Cypate/DOX). 通过马尔文激光散射粒度仪和透射电子显微镜(transmission electron microscope,TEM)考察纳米粒的尺寸大小,进行Zeta电位测定、紫外吸收检测、包封率和载药量的测定、药物体外释放、光热升温等实验,对其性质进一步考察。结果:介孔硅纳米粒尺寸为24.85±0.14 nm,具有明显的介孔结构,Zeta电位表明纳米粒体系稳定。负载药物的MSN-PDA-Cypate/DOX包封率为60.74%,载药量为7.79%(以DOX计算),呈现显著的光热升温能力和一定的靶向性释药。结论:本研究成功制备载Cypate/DOX 介孔硅纳米粒,其稳定性较好,粒径均一,具有较高的药物负载能力和明显的光热升温效应及一定的靶向释药特性,为肿瘤的化疗与光热协同治疗提供了一种策略。
关 键 词: 药剂学;纳米药物;介孔硅纳米粒;聚多巴胺修饰;阿霉素
Title: Study of polydopamine-modified and drug-loaded mesoporous silica nanoparticles
Author: WANG Lu, ZHANG Rui, DENG Yibin, TAO Jing
Organization: College of Pharmaceutical Sciences, Soochow University; The First Affiliated Hospital of Soochow University
Abstract: Objective: To prepare mesoporous silica nanoparticles (MSNs), modified by polydopamine (PDA), loaded with doxorubicin (DOX) and Cypate. Methods: MSNs with hydroxyl group on the surface (MSN-OH) were synthesized by hydrolysis and polymerization of silica-derived tetraethyl orthosilicate (TEOS) in alkaline solution using cetyltrimethylammonium chloride (CTAC) as template. After MSNs being modified by PDA, MSN-NH2-PDA was prepared through HEPES buffer method, and MSNs loaded with Cypate/Dox (MSN-PDA-Cypate/DOX) were prepared through triethylamine desalting method. The morphology of nanoparticles was detected by Malvern laser scattering granulometer and transmission electron microscope (TEM). Zeta potential measurement, UV absorption detection, encapsulation efficiency and drug loading measurement, drug release in vitro and photothermal effects test were conducted respectively to further investigate their properties. Results: The size of the MSNs was 24.85±0.14 nm, which had an obvius mesoporous structure. The Zeta potential indicated that the nanoparticle system was stable. MSN-PDA-Cypate/DOX had an encapsulation rate of 60.74% and drug loading of 7.79% (calculated based on DOX), and exhibited photothermal capacity and targeted drug releasing property. Conclusion: MSNs loaded with Cypate/DOX were successfully prepared. It was found that the stability and uniformity of particle size were good. Besides, the nanoparticles improved drug loading, and also exhibited a significant photothermal capacity and a certain targeted drug releasing property. The research may provide an effective strategy for tumor treatment combined with chemotherapy and photothermal therapy.
Key words: pharmaceutics; nanomedicine; mesoporous silica nanoparticles; polydopamine modification; doxorubicin
发表期数: 2021年9月第3期
引用格式: 王璐,张睿,邓益斌,等. 介孔硅纳米粒的聚多巴胺修饰及药物负载的研究[J]. 中国科技论文在线精品论文,2021,14(3):382-390.
 
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