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丹桂中参与毛蕊花糖苷生物合成的酰基转移酶的挖掘与功能表征

发表时间:2023-09-27  浏览量:188  下载量:21
全部作者: 柴国秀,段炼
作者单位: 武汉大学药学院
摘 要: 为解析桂花中参与毛蕊花糖苷生物合成的酰基转移酶(acyltransferase, AT)和鼠李糖基转移酶(UDP-rhamnosyltransferase , URT)及其催化顺序,本文通过生物信息学分析,从丹桂(Osmanthus tragranscv. Aurantiacu)的转录组中筛选了4个候选AT,并以毛蕊花糖苷的两个重要前体化合物咖啡酰辅酶A和羟基红景天苷(hydroxytyrosol glucoside)为底物进行体外活性测试,确定了可以催化咖啡酰辅酶A与羟基红景天苷反应生成木通苯乙醇苷A的OfAT1。桂花中参与毛蕊花糖苷生物合成的AT的发现为解析和比较毛蕊花糖苷在不同植物中的生物合成方式提供了理论参考。
关 键 词: 生物化学;生物合成;天然产物;毛蕊花糖苷;酰基转移酶
Title: Exploration and functional characterization of acyltransferases from Osmanthus tragranscv. Aurantiacu involved in the biosynthesis of acteoside
Author: CHAI Guoxiu, DUAN lian
Organization: School of Pharmaceutical Sciences, Wuhan University
Abstract: To analyze the acyltransferase (AT) and UDP-rhamnosyltransferase (URT) involved in the biosynthesis of acteoside and their catalytic sequences in Osmanthus fragrans, four candidate ATs from the transcriptome of Osmanthus tragranscv. Aurantiacu were screened through bioinformatics analysis in this paper. Two important precursor compounds of acteoside, caffeoyl coenzyme A and hydroxytyrosol glucoside were taken as substrates to conduct in vitro activity tests, and it was determined that OfAT1 could catalyze the reaction of caffeoyl coenzyme A with hydroxy salidroside to produce calceolarioside A. The discovery of the AT involved in the biosynthesis of acteoside in Osmanthus fragrans provides a theoretical reference for analyzing and comparing the biosynthesis modes of acteoside in different plants.
Key words: biochemistry; biosynthesis; natural products; acteoside; acyltransferase
发表期数: 2023年9月第3期
引用格式: 柴国秀,段炼. 丹桂中参与毛蕊花糖苷生物合成的酰基转移酶的挖掘与功能表征[J]. 中国科技论文在线精品论文,2023,16(3):301-310.
 
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