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农用抗生素KA08活性组分分离及类型分析
发表时间：2013-10-15 浏览量：1546 下载量：603
| 全部作者： | 高芬，李静虹，王梦亮 |
| 作者单位： | 山西大学应用化学研究所 |
| 摘 要： | 采用薄层层析色谱法（thin layer chromatography，TLC），以硅胶GF254为固定相，以乙醇:水=6:4和乙醇:氨水:水=8:1:1（体积比）为展开剂，结合生物追踪测定，分离纯化农用抗生素KA08的活性组分。获得的活性组分IA和IC，经高效液相色谱（high performance liquid chromatography，HPLC）检测，纯度分别为97.53%和98.79%. 通过官能团反应、紫外和红外吸收光谱分析，推测这2种组分均属于氨基糖苷类抗生素，其中，组分IC是一类相对分子量为269.0的小分子化合物。 |
| 关 键 词： | 植物保护学；抗生素KA08；薄层层析；分离纯化；类型分析 |
| Title： | Isolation of active components and analysis of structural types in agro-antibiotic KA08 |
| Author： | GAO Fen, LI Jinghong, WANG Mengliang |
| Organization： | Institute of Applied Chemistry, Shanxi University |
| Abstract： | Thin layer chromatography (TLC) combined with bioassay were applied to the isolation of the active components in agro-antibiotic KA08. The silica gel plate (GF254) was used as the stationary phase, and two mixed solvents, one containing ethanol and water in the volume ratio of 6:4, and the other ethanol, aqueous ammonia and water in the volume ratio of 8:1:1, were used as the developing reagents. The purity of active component IA and IC was 97.53% and 98.79% by high performance liquid chromatography (HPLC) respectively. The functional groups’ reaction, UV and IR spectrum showed that components IA and IC most likely belonged to aminoglycoside antibiotic. Component IC was a small molecule compound with molecular weight of 269.0. |
| Key words： | plant protection; agro-antibiotic KA08; thin layer chromatography; isolation and purification; analysis of structural type |
| 发表期数： | 2013年10月第19期 |
| 引用格式： | 高芬，李静虹，王梦亮. 农用抗生素KA08活性组分分离及类型分析[J]. 中国科技论文在线精品论文，2013，6（19）：1807-1813. |
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