您的位置:首页  > 论文页面

小麦miR166基因家族鉴定及表达分析

发表时间:2024-03-29  浏览量:164  下载量:19
全部作者: 雷晓彤,徐渴,孙若希,张树华,杨学举,赵勇
作者单位: 河北农业大学农学院,华北作物改良与调控国家重点实验室,华北作物种质资源研究与利用教育部重点实验室
摘 要: miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。
关 键 词: 农艺学;Tae-miR166;小麦;进化特征;靶基因
Title: Genome-wide identification and expression analysis of miR166 gene family in whea
Author: LEI Xiaotong, XU Ke, SUN Ruoxi, ZHANG Shuhua, YANG Xueju, ZHAO Yong
Organization: North China Key Laboratory for Crop Germplasm Resources of Education Ministrys, State Key Laboratory of North China Crop, Improvement and Regulation, College of Agronomy, Hebei Agricultural University
Abstract: As an essential post-transcriptional regulator, miR166 played an important role in plant growth and development and response to stress. The evolutionary characteristics and expression patterns of miR166 gene family in wheat (Tae-miR166) were analyzed by PmiREN, Ensembl Plants database, MEGA-X, DNAMAN software, RNAfold web server, WebLogo and psRNATarget online websites. Nineteen members of the Tae-miR166 gene family were searched in the PmiREN database, and chromosome localization revealed that Tae-miR166 were localized on 14 chromosomes. Sequence alignment revealed that the mature sequences of Tae-miR166 were highly conserved; evolutionary analysis showed that the Tae-miR166 gene family members were in 4 evolutionary branches, respectively. The target gene prediction results showed that the target genes of Tae-miR166 gene family included class III homologous heterotypic structural domain leucine zipper protein, β-galactosidase and calcium-dependent protein kinase. RNA-seq data analysis showed that 19 members of the Tae-miR166 family were expressed in 6 tissues of wheat, with the highest expression in grains and ears. The results of RT-qPCR showed that the expression patterns of Tae-miR166 family members were different after cadmium, drought and low temperature stress treatment, indicating that Tae-miR166 gene family played an important role in plant resistance to abiotic stress. This study provided a theoretical basis for further elucidating the function of miR166 gene family in wheat.
Key words: agronomy; Tae-miR166; wheat; evolutionary characteristics; target gene
发表期数: 2024年3月第1期
引用格式: 雷晓彤,徐渴,孙若希,等. 小麦miR166基因家族鉴定及表达分析[J]. 中国科技论文在线精品论文,2024,17(1):41-49.
 
6 评论数 0
暂无评论
友情链接