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应用蛋白质芯片技术从电针胃经大鼠血清中快速筛选低分子量差异蛋白质
发表时间：2008-08-15 浏览量：2102 下载量：840
| 全部作者： | 姚雯，杨宗保，严洁，常小荣，易受乡，叶能胜 |
| 作者单位： | 湖南中医药大学针灸推拿学院；中关村生命科学园北京市蛋白组学研究中心 |
| 摘 要： | 目的：筛选正常大鼠血清与电针胃经大鼠血清中差异表达的低分子量蛋白质，为进一步阐明针刺效应的体液机理提供理论依据。方法：采用表面增强激光解吸离子化飞行时间质谱（SELDI-TOF-MS）技术和弱阳离子交换芯片（WCX2）对正常大鼠血清和电针胃经大鼠血清进行蛋白质指纹图谱检测分析，通过Biomarker Wizard和Biomarker Patterns System软件分析判别处理数据并结合生物信息学方法筛选差异表达蛋白质。结果：与正常大鼠血清比较，电针胃经大鼠血清蛋白质在质荷比为2 000～50 000范围内有25个蛋白质峰差异有显著意义，其中有19个标志蛋白在电针胃经大鼠血清中呈现高表达，6个标志蛋白在电针胃经大鼠血清中呈现低表达。结论：电针胃经大鼠血清与正常大鼠血清中低分子量蛋白质存在明显差异，这种差异蛋白质可能与电针促进胃黏膜损伤修复效应密切相关。 |
| 关 键 词： | 针灸学；表面增强激光解吸离子化飞行时间质谱；电针；胃黏膜损伤；血清蛋白质组 |
| Title： | The rapid detection of low molecular mass proteins differentially expressed in the serum derived from the rats by electroacupuncture at stomach meridian using proteinchip technology |
| Author： | YAO Wen, YANG Zongbao, YAN Jie, CHANG Xiaorong, YI Shouxiang, YE Nengsheng |
| Organization： | School of Acupuncture-Moxibustion and Tui-Na, Hunan University of Chinese Medicine；The Proteomics Research Center of Beijing, Zhongguancun Life Science Park |
| Abstract： | Objective: To detect differentially expressed low mass proteins in the serum between normal rats and impaired gastric mucosal rats by electroacupuncture, and lay a experiment foundation to further explanation of the humoral mechanism of acupuncture effect. Methods: The differentially expressed low mass proteins in the serum were detected by SELDI-TOF-MS and WCX2 proteinchip technology, and primarily analyzed by Biomarker Wizard and Biomarker Patterns System software as well as bioinformatic method. Results: As compared to the normal rat’s serum, the serum of impaired gastric mucosal rat by acupuncture had 25 differentially expressed protein peaks respectively, 19 distinguished proteomic peaks were up-expressed and 6 distinguished proteomic peaks were down-expressed. Conclusion: There are obvious differentially expressed low mass proteins in the serum between normal rats and impaired gastric mucosal rats by electroacupuncture, which maybe have close relationship with the repair effect on gastric mucosal injury by electroacupuncture. |
| Key words： | science of acupuncture and moxibustion; surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; electroacupuncture; gastric mucosal injury; serum proteomic |
| 发表期数： | 2008年12月第15期 |
| 引用格式： | 姚雯，杨宗保，严洁，等. 应用蛋白质芯片技术从电针胃经大鼠血清中快速筛选低分子量差异蛋白质[J]. 中国科技论文在线精品论文，2008，1（15）：1684-1689. |
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