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醋柳黄酮中熊果酸的提取分离与含量测定
发表时间：2008-08-15 浏览量：2254 下载量：874
| 全部作者： | 谭承佳，马家骅，王毓杰，张艺 |
| 作者单位： | 绵阳师范学院生命科学与技术学院；成都中医药大学药学院 |
| 摘 要： | 目的：从醋柳黄酮中提取分离熊果酸并建立其含量测定方法。方法：采用硅胶柱层析法从醋柳黄酮氯仿浸提物中分离得到化合物，经氢核磁共振（1H-NMR），碳核磁共振（13C-NMR）鉴定为熊果酸；采用DiamonsilTM（钻石）C18（250 mm×4�6 mm，5 μm），甲醇-8 mmol·L-1四丁基溴化铵溶液-三乙胺（90∶10∶0�2）为流动相，流速1 mL·min-1，紫外检测波长210 nm，柱温40℃.结果：熊果酸的标准曲线线性范围为0�59～8�85 μg（r=0�999 6，n=5）；平均加样回收率为97�11%，相对标准差（RSD）为1�20%（n=6）。结论：该法简便、准确、重复性好，适用于醋柳黄酮中熊果酸的定量分析。 |
| 关 键 词： | 药物化学；醋柳黄酮；熊果酸；高效液相色谱 |
| Title： | Extraction and determination of ursolic acid from sea-buckthorn flavonoid |
| Author： | TAN Chengjia, MA Jiahua, WANG Yujie, ZHANG Yi |
| Organization： | College of Life Science & Biotechnology, Mianyang Normal University;College of Traditional Chinese Pharmacy, Chengdu University of Traditional Chinese Medicine |
| Abstract： | Objective: To extract ursolic acid from sea-buckthorn flavonoid and determine the content by HPLC. Methods: The compound was obtained from the chloroform extracted fraction of sea-buckthorn flavonoid by column chromatograph. Its structure was identified by 1H-NMR, 13C-NMR as ursolic acid; DiamonsilTM C18(250 mm×4.6 mm, 5 μm) was employed, the mobile phase consisted of methanol -8mmol·L-1 tetrabutyl ammonium bromide-triethylamine (90∶10∶0.2) with a flow velocity of 1.0 mL/min. The detective wavelength was set at 210 nm and the column temperature was at 40℃. Results: The calibration curve was linear in the range of 0.59~8.85 μg (r=0.999 6, n=5), and the average recovery was 97.11%, RSD was 1.20% (n=6). Conclusion: The method is simple and accurate in determining the content of ursolic acid in sea-buckthorn flavonoid. |
| Key words： | pharmaceutical chemistry; sea-buckthorn flavonoid; ursolic acid; high performance liquid chromatography (HPLC) |
| 发表期数： | 2008年12月第15期 |
| 引用格式： | 谭承佳，马家骅，王毓杰，等. 醋柳黄酮中熊果酸的提取分离与含量测定[J]. 中国科技论文在线精品论文，2008，1（15）：1700-1703. |
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