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高效液相色谱法测定叶菜中土霉素的残留量
发表时间：2008-08-31 浏览量：2194 下载量：916
| 全部作者： | 崔馨，乔显亮，王震，韩成伟 |
| 作者单位： | 大连理工大学工业生态与环境工程教育部重点实验室 |
| 摘 要： | 通过Na2EDTA-McIlvaine缓冲液(pH4)超声振荡提取样品的前处理方法，以及优化的高效液相色谱（high performance liquid chromatography, HPLC）条件，建立了3种代表性叶菜（生菜、芹菜和小白菜）中土霉素的定量检测方法。选用的色谱柱为Hypersil BDS C18(250 mm×4.6 mm i.d., 5 μm）反相柱；流动相流速为1.5 mL/min；柱温35℃；检测器为二极管阵列检测器；检测波长为355 nm.由于3种叶菜组织成分存在差异，需采用不同的流动相比例对这3种叶菜中的土霉素进行分离检测。生菜、芹菜和小白菜样品选用的流动相（甲醇∶乙腈∶1%甲酸，pH3.0）体积比分别为10∶15∶75，10∶10∶80，6∶6∶88. 这3种叶菜中土霉素的加标回收率为66.8%～82.9%，相对标准偏差小于10%.该方法具有回收率高和重现性好等优点，可用于叶菜中土霉素残留的检测。 |
| 关 键 词： | 环境科学技术其他学科；土霉素；叶菜；高效液相色谱；提取 |
| Title： | Detection of oxytetracycline residues in leaf-vegetables by high performance liquid chromatography |
| Author： | CUI Xin, QIAO Xianliang, WANG Zhen, HAN Chengwei |
| Organization： | Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, Dalian University of Technology |
| Abstract： | The residues of oxytetracycline in lettuce, celery and pakchoi cabbage were measured with optimum procedure of high performance liquid chromatography (HPLC). Oxytetracycline in the three leaf-vegetable samples was extracted thrice by Na2EDTA-McIlvaine buffer (pH4). The filtrate was examined by HPLC with a reversed phase column Hypersil BDS C18 (250 mm×4.6 mm i.d., 5 μm). The flowing rate of mobile phase was 1.5 mL/min, and the temperature of column was 35℃. The wavelength of DAD detector was 355 nm. Because the three leaf-vegetables are of different compositions, the mobile phase was adjusted to optimum ratio to get satisfactory resolution. The ratios of mobile phase (methanol∶acetonitrile∶1% formic acid, pH3.0) were 10∶15∶75, 10∶10∶80, 6∶6∶88 for lettuce, celery and pakchoi cabbage, respectively. The recovery of oxytetracycline was from 66.8% to 82.9%, and the RSD values of replicates were less than 10%. The results indicated that the method is accurate and stable, which can be used to detect oxytetracycline residues in leaf-vegetables. This analysis procedure will be useful in the study of the uptake of oxytetracycline by vegetables and in the risk assessment of food contamination. |
| Key words： | environmental science and technology to other subjects; oxytetracycline; leaf-vegetable; high performance liquid chromatography （HPLC）; extraction |
| 发表期数： | 2008年12月第16期 |
| 引用格式： | 崔馨，乔显亮，王震，等. 高效液相色谱法测定叶菜中土霉素的残留量[J]. 中国科技论文在线精品论文，2008，1（16）：1778-1783. |
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