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二甲双胍治疗Ⅱ型糖尿病作用机制的代谢组学研究
发表时间：2009-06-15 浏览量：2357 下载量：780
| 全部作者： | 霍韬光，蔡爽，鹿秀梅，熊志立，李发美 |
| 作者单位： | 沈阳药科大学药学院；中国医科大学附属第一医院 |
| 摘 要： | 目的：研究Ⅱ型糖尿病（DM-Ⅱ）患者及给与降糖药二甲双胍治疗后的患者血清代谢物谱变化，从代谢组学的角度阐述二甲双胍治疗Ⅱ型糖尿病的作用机制。方法：采用超高效液相色谱串联质谱（ultra performance liquid chromatography tandem mass spectrometry，UPLC/MS/MS）技术对20名糖尿病患者及15名给与二甲双胍治疗后的患者血清代谢物谱进行分析测定。采用主成分分析（principal components analysis，PCA）和偏最小二乘-判别分析（partial least squares-discriminant analysis，PLS-DA）模型对给与二甲双胍治疗后和未经治疗的Ⅱ型糖尿病患者血清代谢物谱进行分类。结果：二甲双胍治疗后的患者与未经治疗的Ⅱ型糖尿病患者相比血清代谢物谱发生了明显的改变。发现了5个潜在的生物标记物，对药物可能的作用机制进行了讨论。结论：二甲双胍可以调节Ⅱ型糖尿病患者体内代谢紊乱，其作用机制可能与降低血清溶血磷脂酰胆碱有关。 |
| 关 键 词： | 分析化学；生物标记物；超高效液相色谱串联质谱；二甲双胍；Ⅱ型糖尿病 |
| Title： | Metabonomic study on the action mechanism of metformin in the therapy of type Ⅱ diabetes mellitus |
| Author： | HUO Taoguang, CAI Shuang, LU Xiumei, XIONG Zhili, LI Famei |
| Organization： | School of Pharmacy, Shenyang Pharmaceutical University；The First Hospital of China Medical University |
| Abstract： | Objective: To study the metabolic differences between type Ⅱ diabetes mellitus patients and patients treated with metformin and elucidate the action mechanism of metformin from the angle of metabonomic. Methods: Ultra-performance liquid chromatography tandem mass spectrometry (UPLC/MS/MS) was applied to the serum metabolite profiling in type Ⅱ diabetes mellitus (DM-Ⅱ) patients and patients treated with metformin. Principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) models were employed for the discrimination of the two groups. Results: Significant difference in endogenous metabolite profiles was observed in DM-Ⅱ patients and metformin treated patients. Five potential biomarkers were discovered. Possible action mechanism of metformin was discussed. Conclusion: Metformin can regulate the metabolic disorders in DM-Ⅱ patients, which may be associated with its effect to lower serum lysophosphatidylcholines. |
| Key words： | analytical chemistry; biomarkers; UPLC/MS/MS; metformin; type Ⅱ diabetes mellitus |
| 发表期数： | 2009年6月第11期 |
| 引用格式： | 霍韬光，蔡爽，鹿秀梅，等. 二甲双胍治疗Ⅱ型糖尿病作用机制的代谢组学研究[J]. 中国科技论文在线精品论文，2009，2（11）：1157-1162. |
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