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初生乌骨鸡与蛋、肉鸡肝脏组织基因转录阶段的差异探索
发表时间：2009-06-15 浏览量：1923 下载量：594
| 全部作者： | 赵小玲，李亮，肖蔹，朱庆 |
| 作者单位： | 四川农业大学动物科技学院 |
| 摘 要： | 目的：探索中国珍贵的家禽品种资源——丝羽乌骨鸡和四川山地乌骨鸡特异性状相关蛋白的表达和调控机制。方法：以蛋鸡和肉鸡为对照，结合银染差异显示逆转录聚合酶链式反应（differential display reverse transcription polymerase chain reaction，DDRT-PCR）技术和基因芯片技术筛选1日龄乌骨鸡肝脏组织差异显示的表达序列标签（expressed sequence tag, EST)。结果：筛选到3条真实的差异表达cDNA序列。DQ003271仅在蛋鸡中表达，为家鸡新的EST，位于5号染色体；DQ003272仅在乌骨鸡中表达，为家鸡已知EST，属于Gga.9136基因簇，contig 1的正向阅读框1序列与NP_001012582.1一致性达91%，推测该片断所在的基因极可能直接影响酪氨酸酶的表达，控制“乌色”性状；DQ003274未在山地乌骨鸡中表达，与鸡肠淋巴细胞cDNA克隆序列CD737238.1一致性达100%，属基因簇Gga.8993.结论：为进一步研究乌骨鸡的独特性状及乌骨鸡、蛋鸡、肉鸡三者间的差异性状提供了重要信息。 |
| 关 键 词： | 动物遗传育种；mRNA差异显示聚合酶链式反应；乌骨鸡；表达序列标签；基因芯片 |
| Title： | Study on genetic transcription difference of liver tissues of nascent black-bone chicken, layer and broiler |
| Author： | ZHAO Xiaoling, LI Liang, XIAO Lian, ZHU Qing |
| Organization： | Department of Animal Science and Technology, Sichuan Agricultural University |
| Abstract： | Objective: To study expression and regulation mechanisms of proteins related with specific characteristics in Silky fowl and Sichuan mountain black-bone chicken. Methods: Differentially display reverse transcription polymerase chain reaction (PCR) and gene chip scanning were adopted to attain expressed sequence tag (EST) which were differentially displayed among the black-bone chicken, the layer and the broiler. Results: Three ESTs were obtained. Bioinformation analysis proved that DQ003271 only expressed in the layer was a new EST, located at the fifth chromosome; and DQ003272 only expressed in the black-bone chicken was a known EST, belonging to Gga.9136. The first sequence of positive reading frame of contig 1 was 91% homologous to NP_001012582.1. The gene including the segment may directly control the expression of Tyrosinase, thereby controlling the black color trait. DQ003274 only not expressed in the Sichuan mountain black-bone chicken had 100% consistency with CD737238.1 and belonged to Gga.8993. Conclusion: These results provide significant data for further research on different traits among the black-bone chicken, the layer and the broiler. |
| Key words： | animal genetics and breeding; mRNA differential display polymerase chain reaction; black-bone chicken; expressed sequence tags; gene chip |
| 发表期数： | 2009年6月第11期 |
| 引用格式： | 赵小玲，李亮，肖蔹，等. 初生乌骨鸡与蛋、肉鸡肝脏组织基因转录阶段的差异探索[J]. 中国科技论文在线精品论文，2009，2（11）：1208-1214. |
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