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表皮生长因子受体及基质金属蛋白酶-2在食管癌的表达特点及意义
发表时间：2010-06-15 浏览量：1780 下载量：579
| 全部作者： | 刘敏，郭晓娟，金木兰，郭佑民，段小艺 |
| 作者单位： | 首都医科大学附属北京朝阳医院介入放射科；首都医科大学附属北京朝阳医院病理科；西安交通大学医学院第二附属医院放射科；西安交通大学医学院第一附属医院影像中心 |
| 摘 要： | 目的：探讨食管癌组织中表皮生长因子受体（epidermal growth factor receptor，EGFR）和基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）的表达关系。方法：应用免疫组织化学及蛋白印迹实验检测66例人食管癌组织及瘤旁正常组织EGFR及MMP-2的表达，评价EGFR及MMP-2在患者性别、年龄、肿瘤TNM（tumor，lymph node，metastasis）分期、病理分级等临床参数组的表达。Pearson相关性检验分析EGFR及MMP-2表达相关性。结果：免疫组化显示EGFR，MMP-2在肿瘤组织表达的平均灰度净值分别为25.42±3.15，37.46±6.18，在瘤旁正常食管组织平均灰度净值分别为5.03±3.49，9.80±2.24，组织间表达水平相比均有显著的统计学差异（P＜0.01）。免疫组化及蛋白印迹表达分析显示EGFR，MMP-2的表达分布在TNM分期、病理分级和淋巴结转移中均有统计学差异（P<0.05）。免疫组化检测两蛋白表达净灰度值相关系数R=0.425，P=0.038；蛋白印迹检测2种蛋白表达相关系数R=0.674，P<0.05.结论：EGFR及MMP-2在食管癌均存在过表达，且二者的表达呈正相关，显示EGFR及MMP-2与食管癌发生、发展密切相关，为食管癌多靶点联合治疗提供依据。 |
| 关 键 词： | 临床病理学；表皮生长因子受体；基质金属蛋白酶-2；食管癌 |
| Title： | Expression and correlation of epidermal growth factor receptor and matrix metalloproteinase-2 in human esophageal carcinoma |
| Author： | LIU Min, GUO Xiaojuan, JIN Mulan, GUO Youmin, DUAN Xiaoyi |
| Organization： | Department of Radiology, Beijing Chaoyang Hospital, Capital Medical University；Department of Pathology, Beijing Chaoyang Hospital, Capital Medical University；Department of Radiology, the Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University；Imaging Center, the First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University |
| Abstract： | Objective: To detect the expression and relation of epidermal growth factor receptor (EGFR) and matrix metalloproteinase-2 (MMP-2) in human esophageal carcinoma. Methods: In 66 human esophageal carcinoma tissues, the expressions of EGFR and MMP-2 were detected by immunohistochemistry and Western-blot. The expressions of EGFR and MMP-2 in various clinicopathologic analyzed. Correlation analysis between MMP-2 and EGFR was analyzed by Pearson correlation coefficient. Results: By immunohistochemistry, the average gray scale values of EGFR in esophageal carcinoma and normal tissues were 25.42±3.15, 5.03±3.49, which showed the significant difference (P＜0.01). And the average gray scale values of MMP-2 in esophageal carcinoma and normal tissues were 37.46±6.18 and 9.80±2.24, which showed a significant difference (P＜0.01). The significant differences were observed in TNM-stage, lymph node metastasis and tumor classification in both the expression of EGFR (P<0.05) and MMP-2 (P<0.05) by immunohistochemistry and Western-blot. Correlation analysis between EGFR and MMP-2 by immunohistochemistry (R=0.425, P=0.038) and Western-blot respectively(R=0.674, P<0.05) suggested a good positive correlation. Conclusion: The data suggests that EGFR and MMP-2 overexpress in human esophageal carcinoma. Combination of EGFR and MMP-2 could be the useful markers for tumorigenesis and differentiation, which provides basis for therapy of human esophageal carcinoma. |
| Key words： | clinical pathology; epidermal growth factor receptor; matrix metalloproteinase-2; esophageal carcinoma |
| 发表期数： | 2010年6月第11期 |
| 引用格式： | 刘敏，郭晓娟，金木兰，等. 表皮生长因子受体及基质金属蛋白酶-2在食管癌的表达特点及意义[J]. 中国科技论文在线精品论文，2010，3（11）：1107-1112. |
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